Protein Info for LRK54_RS11290 in Rhodanobacter denitrificans FW104-10B01

Annotation: dihydrolipoyllysine-residue acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01348: dihydrolipoyllysine-residue acetyltransferase
  • amino acids 3 to 460 (458 residues), 629.9 bits, E=2.1e-193
• PF00364: Biotin_lipoyl
  • amino acids 6 to 76 (71 residues), 74.4 bits, E=1.3e-24
• PF01597: GCV_H
  • amino acids 23 to 66 (44 residues), 23 bits, 1.6e-08
• PF02817: E3_binding
  • amino acids 154 to 188 (35 residues), 57.8 bits, 2.4e-19
• PF00198: 2-oxoacid_dh
  • amino acids 228 to 460 (233 residues), 269 bits, E=8.2e-84

Best Hits

KEGG orthology group: K00627, pyruvate dehydrogenase E2 component (dihydrolipoamide acetyltransferase) [EC: 2.3.1.12] (inferred from 62% identity to psu:Psesu_0310)

Predicted SEED Role

"Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 2.3.1.12)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• pyruvate fermentation to acetate V (3/3 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (3/4 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (17/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.12

Protein Sequence (460 amino acids)

>LRK54_RS11290 dihydrolipoyllysine-residue acetyltransferase (Rhodanobacter denitrificans FW104-10B01)
MADLKEARVPDIGHADVPVIEVLVKAGDRVEKEQSLITLESDKATMEVPAPFAGTVKEVR
LKVGDEVSEGAVIAIIETDGAAAEPAAAKPAAAPAPAAPAPASVPAPPATAPASAEPKPA
PIAGQGVHNAPEGDVPPQARPPFDANIVMPGDAPYASPAVRAFARELGVDIQQVKGSGRG
GRIQREDVSAYVKHALASGARPVVGAAASVGGLNLLPWPKVDFAKFGEIEERPLSRIQKI
SGANLARNWAMIPHVTQHEDADITEMEAFRKQLGAEQKSDKGGDLKISPLVFQIKAVVAA
LKQFPQFNASLDESGEKLILKKYFHIGIAVDTPDGLVVPVIRDCDKKGLLDLARDLAEIS
KKARDKKLGPAEMSGGCFSISSLGGIGGTYFTPIVNAPEVAILGVSKSVMKPVWNGKEFA
PRLLLPLSLSYDHRVIDGALAARFASFLANQLGDIRRLLL