Protein Info for LRK54_RS11075 in Rhodanobacter denitrificans FW104-10B01

Annotation: primosomal protein N'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 724 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF17764: PriA_3primeBD" amino acids 6 to 101 (96 residues), 83.5 bits, see alignment E=2.4e-27 PF04851: ResIII" amino acids 193 to 324 (132 residues), 51.2 bits, see alignment E=4.2e-17 PF00270: DEAD" amino acids 211 to 359 (149 residues), 57.2 bits, see alignment E=5.5e-19 TIGR00595: primosomal protein N'" amino acids 216 to 721 (506 residues), 587.5 bits, see alignment E=1.1e-180 PF18319: PriA_CRR" amino acids 440 to 466 (27 residues), 38.5 bits, see alignment (E = 2.6e-13) PF00271: Helicase_C" amino acids 486 to 583 (98 residues), 29.4 bits, see alignment E=2.5e-10 PF18074: PriA_C" amino acids 627 to 720 (94 residues), 72.5 bits, see alignment E=1.3e-23

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 56% identity to psu:Psesu_2763)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (724 amino acids)

>LRK54_RS11075 primosomal protein N' (Rhodanobacter denitrificans FW104-10B01)
MPAVLRVALPVPLPGLFDYLPPTVGAACVGSRVLVPFGRHKLVGMVVTIDAEAAVGSSRL
KQVLRLLDDGALLDPELMQTLAWAADYWLGAPGEAYANALPLALREAKPLPPIGDEYWSL
SGAGRSAHDAGSRRGGSKALLDLLAAGALSAQELSERQPGWREAARRLATAGLLERSERP
PPDRSSPPSLAPQLSDEQQQAVVTVGASLGQYQPFLLDGVTGSGKTEVYLALIEQVLAQG
RQALLLVPEIGLAPQTMRRLRERLGVIVEVLHSNLSEGDRARAWLRARAGSARVILGTRS
AVFTPLPQAGLIIVDEEHDSAYKQQEGFRYHARDLALVRARALGVPVLLGSGTPSLESLA
NAEAGRYRTLHLRARPGAIRPPQVQIIDMRAQRLEHGMSPALLGAVADTVARGEQALVFR
NRRGYAPVLLCHACGWHAGCPRCDRPLTLHAGRRQLICHHCDYRQRVPAACPTCGAGDLK
PQGQGTERLEEALLAQFPQVPVLRVDRETTRRRDAFEQLLATLRDDQPAILVGTQMLAKG
HDLPNLTLVAIVGVDEGLHSIDFRAGERLAQLVVQVAGRAGRARKPGRVLLQTHQPEHPL
LRSLLAHGYAAAARELLAERRLIQLPPYSHQVLLRAEAPQREQVDAFLAAAHAALPVNDQ
LQIAGPMPAPMPLRAGRHRGQLLLEAASRRTLHGRLRSWQLALIALPSARKVRWSLDVDP
IDLY