Protein Info for LRK54_RS04330 in Rhodanobacter denitrificans FW104-10B01

Annotation: valine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 942 transmembrane" amino acids 498 to 518 (21 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 1 to 923 (923 residues), 1078 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 625 (612 residues), 435.5 bits, see alignment E=4.8e-134 PF09334: tRNA-synt_1g" amino acids 40 to 86 (47 residues), 25 bits, see alignment 2.1e-09 PF13603: tRNA-synt_1_2" amino acids 238 to 293 (56 residues), 29 bits, see alignment 1.9e-10 PF08264: Anticodon_1" amino acids 667 to 816 (150 residues), 133.7 bits, see alignment E=1.2e-42 PF10458: Val_tRNA-synt_C" amino acids 877 to 942 (66 residues), 60.3 bits, see alignment 4.7e-20

Best Hits

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (942 amino acids)

>LRK54_RS04330 valine--tRNA ligase (Rhodanobacter denitrificans FW104-10B01)
MDKSFEPARIESTWYARWEASGAFRPSGQGEPYCILLPPPNVTGTLHMGHAFQQTVMDML
VRYHRMRGFNTLWQVGTDHAGIATQKIVENQLAAEDKTRHDLGRDAFVERVWQWKEASGS
TITNQMRRIGAAADWSRERFTMDEGLSAAVRKVFIDWYRAGLIYRGNRLVNWDPVLGTAV
SDLEVNNVERDGHLWSIRYTVTGSDDSLVVATTRPETMLGDVAVAVHPEDERYAHLVGKT
LKLPLTDREIPVIADDYVDKDFGTGCVKITPAHDFNDYAIGQRHQLPPITIFTLDAKVND
NAPPKYRGLDRYDARKAVLADLEASGLLVETKPHKLQVPVSQRSDAVIEPMLTDQWFVDL
TSDVQKDGRPGGRKAITQPALDAVRNGDIKFVPENWATTYTQWLDNIQDWCISRQLWWGH
RIPAWYDEAGNIFVGEDEADARASAGTAPVGALRQDDDVLDTWFSSALWPFSTLGWPANG
PVKNERGEIVANWEQDQIFLPSAVLVTGFDIIFFWVARMVMATKYFTGRIPFREVYINAI
VRDAEGQKMSKSKGNTLDPLDLIDGIALEPLVEKSTRSLLIPQVRAKVEKRIRKDYPDGI
PAIGTDALRFTFAALASYSRTINFDIKRAEGYKAFCNKLWNAARFVLMNLPEGAIAAPAG
APVTEAERWILTRLKQTLGEVEQHFVSYRFDLLAQALYEFVWNEYCDWFLELSKPALNGD
DTAAATSARHTLLVVLESVLRALHPVIPFITEEVWASVAPMLGLGEGSLMQRPWPRAEDI
VADDAATAEIEWFKNVLSGVRKIRSEMNISPGKTIPLLLAGGDAGDRARVAKFAAQIAFL
ARTEAPWWIEAGAAEPAAAAAVVGTLRVLIPLAGLIDLGAEKTRLAKEIARIEVEIRKCE
GKLGNASFVANAPAEVVAQERQRVADWNSTLGALREQAQKLG