Protein Info for LRK54_RS01490 in Rhodanobacter denitrificans FW104-10B01

Annotation: DNA topoisomerase IV subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 629 TIGR01055: DNA topoisomerase IV, B subunit" amino acids 4 to 624 (621 residues), 949.8 bits, see alignment E=3.4e-290 PF02518: HATPase_c" amino acids 28 to 168 (141 residues), 61.5 bits, see alignment E=2.1e-20 PF00204: DNA_gyraseB" amino acids 218 to 383 (166 residues), 138.9 bits, see alignment E=2.5e-44 PF01751: Toprim" amino acids 412 to 520 (109 residues), 51 bits, see alignment E=2.7e-17 PF00986: DNA_gyraseB_C" amino acids 557 to 619 (63 residues), 71.9 bits, see alignment E=8e-24

Best Hits

Swiss-Prot: 70% identical to PARE_PSEAE: DNA topoisomerase 4 subunit B (parE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02622, topoisomerase IV subunit B [EC: 5.99.1.-] (inferred from 79% identity to psu:Psesu_0890)

Predicted SEED Role

"Topoisomerase IV subunit B (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (629 amino acids)

>LRK54_RS01490 DNA topoisomerase IV subunit B (Rhodanobacter denitrificans FW104-10B01)
MSSRYNAADIEVLSGLDPVKRRPGMYTDTTRPNHLVQEVVDNSVDEALAGHAKTIEVVLH
DDGSVEVSDDGRGMPVDIHPEEGVSGVELIMTRLHAGGKFSGKNYNFSGGLHGVGVSVVN
ALSDRVEVTIRRDGSEYRMVFAHGDKISPLEVIGTVPKKRSGTTLRFWPEAKYFDSPKIS
LGKLKHLLRAKAVLCAGLNVKLTDVASGEVVEWHYEDGLRDYLRGELDGIEALPNELFVH
QMARDIEGMEVALAWLPEGELVQESYVNLIPTAQGGTHVNGLRTGLTTAIREFCDIRNLL
PRGVKLAPEDVWERLSFVLSTRLQDPQFAGQTKERLSSRQTAGMVENVIHDAFSLWLNQH
VEMGERIAQLAIERASARLKAAKQIVRKKITQGPQLPGKLADCTATDLTRTELFLVEGDS
AGGSAKQARDKEFQAILPLRGKILNTWEVESTSVLASEEVHNLAVAIGCDPGKDDLSGLR
YGKVIILADADSDGLHIATLLSALFLRHFPTLVSEGHVFVAMPPLFRVDVGKQVFYCLDE
AEKEAMLQRIEREKMKGAVSTTRFKGLGEMNPPQLRESTIHPDTRRLVQLTVDDGDGTAR
IMDLLLAKKRAGDRKSWLEEKGDLATLEV