Protein Info for LRK54_RS00290 in Rhodanobacter denitrificans FW104-10B01

Annotation: lysine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 510 TIGR00499: lysine--tRNA ligase" amino acids 17 to 509 (493 residues), 656.6 bits, see alignment E=1.2e-201 PF01336: tRNA_anti-codon" amino acids 73 to 149 (77 residues), 56.2 bits, see alignment E=2.8e-19 PF00152: tRNA-synt_2" amino acids 166 to 506 (341 residues), 308 bits, see alignment E=7.3e-96

Best Hits

Swiss-Prot: 77% identical to SYK_XANOM: Lysine--tRNA ligase (lysS) from Xanthomonas oryzae pv. oryzae (strain MAFF 311018)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 77% identity to xoo:XOO2872)

MetaCyc: 58% identical to lysyl-tRNA synthetase (Escherichia coli K-12 substr. MG1655)
Lysine--tRNA ligase. [EC: 6.1.1.6]; 2.7.7.- [EC: 6.1.1.6]; 2.7.7.- [EC: 6.1.1.6]; RXN-23921 [EC: 6.1.1.6]; RXN-23924 [EC: 6.1.1.6]; RXN-23927 [EC: 6.1.1.6]; RXN0-7397 [EC: 6.1.1.6]

Predicted SEED Role

"Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.6

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (510 amino acids)

>LRK54_RS00290 lysine--tRNA ligase (Rhodanobacter denitrificans FW104-10B01)
MSEATDTQPVDENKPDVNRLVAERREKLKALRGHGIAFPNDFKVDSFAGDLQAEFADKDV
HTAEAVEAAVRRVKMAGRIVLKRVQGKVSFVQLQDFSGRIQLFIHQGTLGEAYEAFKGWD
VGDIVGAEGVLMRTKTGELSVRVDRLRLLTKSLRPLPDKFHGLADVEQRYRQRYVDLIVT
EEARRTFMLRSKIIGFMRQWLEAPSRRFMEVETPMMHVIPGGATAKPFVTHHNALDLDLY
LRVAPELYLKRLVVGGFDRVYEINRNFRNEGVSTRHNPEFTMLELYQAYATYHEIMDLTE
AVIRDTAQAVLGTTQLEWDGAAIDVGPTFRRWRMEDAVLEHNPGIKREELRDRAAMAAHA
KRLGVQVKDNYGWGKLLLEIFEKTVEHTLIQPTFITDHPVEVSPLARESDTDKGITDRFE
LFVGGKEIANGFSELNDPEDQAARFQAQVDAKDAGDDEAMHFDADYIRALEVGLPPTGGL
GIGIDRLVMLLTGSSSIRDVLLFPYMRPEA