Protein Info for IAI47_08125 in Pantoea sp. MT58

Annotation: alpha-amylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 493 PF00128: Alpha-amylase" amino acids 25 to 369 (345 residues), 79.5 bits, see alignment E=3.8e-26

Best Hits

Swiss-Prot: 74% identical to AMY2_ECOLI: Cytoplasmic alpha-amylase (amyA) from Escherichia coli (strain K12)

KEGG orthology group: K01176, alpha-amylase [EC: 3.2.1.1] (inferred from 80% identity to ebi:EbC_26420)

MetaCyc: 74% identical to alpha-amylase (Escherichia coli K-12 substr. MG1655)
Alpha-amylase. [EC: 3.2.1.1]

Predicted SEED Role

"Cytoplasmic alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (493 amino acids)

>IAI47_08125 alpha-amylase (Pantoea sp. MT58)
MQNPTLLQFFHWYYPDGSKLWPEVAERAAWLSEIGISMVWLPPCYKGGSGGYSVGYDSYD
LFDLGEFDQKGGVATKYGDKTQLLAAAEALRSHNVGVLLDVVLNHKMGADEKEAISVNRV
NPDNRDEIYEEVVECEAWTKFTFPARAGEYSKFVWDHKCFSGVDHIENPDENGVFKIIND
YTAEGWNDQVDNELGNFDYLMGANIDFRNNAVREELKYWARWVMEQVPCTGFRLDAVKHI
PAWFYKEWIEHIQEVAEEPMFIVAEYWSFETEKLQEYVHQVEGKTMLFDAPLHMNFHQAS
TAGSAYDMSQIFANSLVVADPWHAVTIVANHDTQPLQSLEAPVEAWFKPLAYALILLREQ
GVPTIFYPDLFGATYEDEGGDGETHKIEMPVIPELEGLIRARQQYGWGVQTDYFDHPNCV
AFSRSGTETQPGCVVIMSNGDAGEKSVPMGEGFAGKVWRDYLGNREETITADEHGTAVFA
CNGGSVSVWVIAE