Protein Info for HSERO_RS23440 in Herbaspirillum seropedicae SmR1

Annotation: isovaleryl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 394 PF02771: Acyl-CoA_dh_N" amino acids 13 to 124 (112 residues), 143.8 bits, see alignment E=5.5e-46 PF02770: Acyl-CoA_dh_M" amino acids 128 to 223 (96 residues), 96.2 bits, see alignment E=2.2e-31 PF00441: Acyl-CoA_dh_1" amino acids 235 to 389 (155 residues), 132 bits, see alignment E=4.2e-42 PF08028: Acyl-CoA_dh_2" amino acids 257 to 372 (116 residues), 59.6 bits, see alignment E=7.9e-20

Best Hits

Swiss-Prot: 66% identical to IVD_BOVIN: Isovaleryl-CoA dehydrogenase, mitochondrial (IVD) from Bos taurus

KEGG orthology group: K00253, isovaleryl-CoA dehydrogenase [EC: 1.3.99.10] (inferred from 100% identity to hse:Hsero_4687)

MetaCyc: 73% identical to isovaleryl-CoA dehydrogenase subunit (Pseudomonas aeruginosa PAO1)
RXN0-2301 [EC: 1.3.8.4]

Predicted SEED Role

"Isovaleryl-CoA dehydrogenase (EC 1.3.8.4)" (EC 1.3.8.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.4

Use Curated BLAST to search for 1.3.8.4 or 1.3.99.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IY31 at UniProt or InterPro

Protein Sequence (394 amino acids)

>HSERO_RS23440 isovaleryl-CoA dehydrogenase (Herbaspirillum seropedicae SmR1)
MIHLPGLSFDHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGIT
VSEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRL
ISGDFIGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDL
EAGARGMTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNV
LMSGLDFERSVLSGGPLGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMA
CKAYVYAVGQACDRADSADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNGYINEYP
VGRLWRDAKLYEIGAGTSEIRRMLIGRELFADTL