Protein Info for HSERO_RS22235 in Herbaspirillum seropedicae SmR1

Updated annotation (from data): rhamnulose-1-phosphate aldolase (EC 4.1.2.19) / lactaldehyde dehydrogenase (EC 1.2.1.22)
Rationale: Specific phenotype: utilization of L-Rhamnose
Original annotation: short-chain dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02632: rhamnulose-1-phosphate aldolase/alcohol dehydrogenase
  • amino acids 18 to 690 (673 residues), 1058.4 bits, E=0
• PF00596: Aldolase_II
  • amino acids 28 to 217 (190 residues), 62.8 bits, E=9.9e-21
• PF00106: adh_short
  • amino acids 430 to 613 (184 residues), 153.7 bits, E=1.1e-48
• PF08659: KR
  • amino acids 432 to 593 (162 residues), 41.7 bits, E=3e-14
• PF13561: adh_short_C2
  • amino acids 439 to 683 (245 residues), 159.4 bits, E=3e-50

Best Hits

KEGG orthology group: None (inferred from 100% identity to hse:Hsero_4449)

Predicted SEED Role

"Predicted rhamnulose-1-phosphate aldolase (EC 4.1.2.19) / Predicted lactaldehyde dehydrogenase (EC 1.2.1.22)" in subsystem L-rhamnose utilization (EC 1.2.1.22, EC 4.1.2.19)

MetaCyc Pathways

• L-rhamnose degradation I (4/5 steps found)
• methylglyoxal degradation V (2/3 steps found)
• L-lactaldehyde degradation (aerobic) (1/2 steps found)
• methylglyoxal degradation IV (1/3 steps found)
• lactate biosynthesis (archaea) (2/5 steps found)
• superpathway of methylglyoxal degradation (4/8 steps found)
• superpathway of fucose and rhamnose degradation (6/12 steps found)
• L-rhamnose degradation II (2/8 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Pentose and glucuronate interconversions
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.22

Use Curated BLAST to search for 1.2.1.22 or 4.1.2.19

Sequence Analysis Tools

See D8IVV7 at UniProt or InterPro

Protein Sequence (690 amino acids)

>HSERO_RS22235 rhamnulose-1-phosphate aldolase (EC 4.1.2.19) / lactaldehyde dehydrogenase (EC 1.2.1.22) (Herbaspirillum seropedicae SmR1)
IASAWDEAYAATLDEPQLLLYRSNLLGADKEITNFGGGNTSAKIAMPDPLTGQTVEVLWV
KGSGGDLGSIKLDGFATLYMDKLEALRSRYRGLEHEDEMVGYLPHCTYNLNARAASIDTP
LHAYIPRRHVDHMHPDAVIAIAACKNSRDLTARIFEGELGWLPWQRPGYDLGLKLEQLVR
AQPHLKGIVLEGHGLFTWGDSARECYENTLNVIRRADAWLAANSRTPAFGGARYQALPKA
ERAALAARIMPLLRGKISKGEYKLGHFDDSQAVLDFVCSNDLAALAALGTSCPDHFLRTK
IRPLVLDFSPQQPDLAALEASLDAALEAYRQDYAAYYQRCARPNSPAMRDPNPVIYLIPG
VGMLSFAKDKATARIAGEFYINAVNVMRGANGVDSYVGLPEQEAFDIEYWLLEEAKLQRM
PKPKSLAGRIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQSALDSAQQSLLKHGGPDA
IGVVRADITSEADVTTILNSVALRFGGIDLLVSNAGIASASPLQDTSLEVWQRNLSVLAT
GYFLVSRAAFSSMLTQGLGGSIVYVASKNGLVASAGASAYCTAKAAEIHLARCIALEGAP
HGIRVNVVNPDAVIRGSRIWDGKWKEERAASNKIEADDVEEFYRQRSMLKRSVLPEDIAE
AVYFFASEKSAKSTGNILNVDAGNAGAFTR