Protein Info for HSERO_RS22065 in Herbaspirillum seropedicae SmR1

Annotation: EpiA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 317 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF04321: RmlD_sub_bind" amino acids 8 to 155 (148 residues), 30 bits, see alignment E=5.7e-11 PF01370: Epimerase" amino acids 8 to 230 (223 residues), 205.3 bits, see alignment E=2.1e-64 PF02719: Polysacc_synt_2" amino acids 39 to 107 (69 residues), 20.6 bits, see alignment E=4.6e-08 PF16363: GDP_Man_Dehyd" amino acids 40 to 303 (264 residues), 66.6 bits, see alignment E=5.8e-22

Best Hits

Swiss-Prot: 44% identical to FCL_SINFN: GDP-L-fucose synthase (fcl) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K02377, GDP-L-fucose synthase [EC: 1.1.1.271] (inferred from 100% identity to hse:Hsero_4414)

Predicted SEED Role

"GDP-L-fucose synthetase (EC 1.1.1.271)" in subsystem Capsular heptose biosynthesis or Colanic acid biosynthesis (EC 1.1.1.271)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.271

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IVS3 at UniProt or InterPro

Protein Sequence (317 amino acids)

>HSERO_RS22065 EpiA (Herbaspirillum seropedicae SmR1)
MPSRDASIFVAGHAGLIGSAILRALQQAGYRKLITRTRAQLELRDKQQVDAFFEAERPDY
VMLAAGKVGGILENQSFPADFINANLAISLNVLEAAHRCGVKRLVMFGSSCMYPRETAQP
MAETALLSGHPEPTSLPYAIAKLAGVQLCLAYNRQYGQQRFIPLIPNSAYGPNDNFDPKS
GHVLSSLMARFHQAKLDGAEAVTLWGSGTPRREFIHADDIAAAALLMMREQLPALTLPLN
VGSGSDISIRELAQAIAGVVGYEGRLEWDSSKPDGALRKLLDSSLMHQQGWRPQVGFAEG
LRDTYAWYVQNKAGRAV