Protein Info for HSERO_RS21670 in Herbaspirillum seropedicae SmR1

Annotation: tRNA modification GTPase TrmE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 466 PF10396: TrmE_N" amino acids 8 to 125 (118 residues), 133.6 bits, see alignment E=8.2e-43 TIGR00450: tRNA modification GTPase TrmE" amino acids 12 to 466 (455 residues), 319.5 bits, see alignment E=4.1e-99 PF12631: MnmE_helical" amino acids 128 to 463 (336 residues), 188.9 bits, see alignment E=2.7e-59 TIGR00231: small GTP-binding protein domain" amino acids 223 to 373 (151 residues), 73.9 bits, see alignment E=1.3e-24 PF01926: MMR_HSR1" amino acids 224 to 343 (120 residues), 83.9 bits, see alignment E=1.9e-27 PF02421: FeoB_N" amino acids 224 to 316 (93 residues), 33 bits, see alignment E=8.2e-12

Best Hits

Swiss-Prot: 77% identical to MNME_HERAR: tRNA modification GTPase MnmE (mnmE) from Herminiimonas arsenicoxydans

KEGG orthology group: K03650, tRNA modification GTPase (inferred from 100% identity to hse:Hsero_4335)

MetaCyc: 51% identical to 5-carboxymethylaminomethyluridine-tRNA synthase GTPase subunit (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"GTPase and tRNA-U34 5-formylation enzyme TrmE" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IUS1 at UniProt or InterPro

Protein Sequence (466 amino acids)

>HSERO_RS21670 tRNA modification GTPase TrmE (Herbaspirillum seropedicae SmR1)
MTFDSSPIAAIATAPGRGGIGVVRLSGKNLAPVIQAVCGRASLAPRHATYLPFLNEDGSA
IDQGLAIHFPAPHSYTGEDVLELQGHGGPVVMQMLLARCVQAGSEIGLRLAEPGEFTQRA
FLNDKIDLAQAEAVADLIEATTEAAAKSASESLSGAFSKVIHELVDQVTRLRMLVEATLD
FPEEEIDFLKQSDARGQLATIRSTLEEVFKHAAQGALLRDGLNAVLAGKPNVGKSSLLNV
LAGSDVAIVTPIAGTTRDKVTQTIQIEGMPLNIIDTAGIREAGDDGSGPDEVERIGIERT
WAEVAKADVILHMLDADRGPTLEDEKITSRFPDGVPIIRIWNKIDRSGHKAAVDKMPDAT
HVYLSASEGQGVDLLRAELLRIAGWQQTVESRYLARERHLLALKAADEHLAHAAAHAALD
NEAGEHALDLFAEELRLAQERLSSITGKFTPDDLLGVIFSRFCIGK