Protein Info for HSERO_RS21635 in Herbaspirillum seropedicae SmR1

Annotation: DNA-directed DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 384 PF00817: IMS" amino acids 10 to 174 (165 residues), 137.2 bits, see alignment E=8.6e-44 PF11798: IMS_HHH" amino acids 186 to 217 (32 residues), 36.9 bits, see alignment (E = 5.4e-13) PF14520: HHH_5" amino acids 199 to 233 (35 residues), 28.6 bits, see alignment 3.2e-10 PF11799: IMS_C" amino acids 261 to 368 (108 residues), 96.7 bits, see alignment E=2.2e-31

Best Hits

Swiss-Prot: 41% identical to DPO4_SYMTH: DNA polymerase IV (dinB) from Symbiobacterium thermophilum (strain T / IAM 14863)

KEGG orthology group: K02346, DNA polymerase IV [EC: 2.7.7.7] (inferred from 100% identity to hse:Hsero_4328)

Predicted SEED Role

"DNA polymerase IV (EC 2.7.7.7)" in subsystem DNA repair, bacterial (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IUR5 at UniProt or InterPro

Protein Sequence (384 amino acids)

>HSERO_RS21635 DNA-directed DNA polymerase (Herbaspirillum seropedicae SmR1)
MANDRRIAHLDMDAFYASVELLRYPDLRGQAVVIGGGSATRPVELPDGTRQYFRMRDYAG
RGVVTTSTYEARALGVFSAMGIMKAAQLAPDAILLPTDFEAYRKYSRLFKEAVRNIAPLI
EDRGIDEIYIDLSAWSDTAPEVARQIKDAVHAATSLTCSIGVAPNKMLAKISSELDKPNG
LTILTPADIERRIWPLPVRKINGIGPKAAEKLSALGIDTVADLAGAAPDLLRAHFGRSYA
EWLGRVAQGVDDRPVQTYSEPKSISRETTFERDLHARADRAQLSEIFTALCVKLAADLDR
KGYVGRTIGIKLKYADFRGVTRDVTLPSPTGDAAAIRQAAGECLKRVPLDKKLRLLGVRV
GALSKKDEQAAASHAVQAELPFAS