Protein Info for HSERO_RS21390 in Herbaspirillum seropedicae SmR1

Annotation: phenylalanine 4-monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 TIGR01267: phenylalanine-4-hydroxylase" amino acids 34 to 277 (244 residues), 341.8 bits, see alignment E=9.4e-107 PF00351: Biopterin_H" amino acids 43 to 257 (215 residues), 156.2 bits, see alignment E=5.6e-50

Best Hits

Swiss-Prot: 60% identical to PH4H_CHRVO: Phenylalanine-4-hydroxylase (phhA) from Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)

KEGG orthology group: K00500, phenylalanine-4-hydroxylase [EC: 1.14.16.1] (inferred from 100% identity to hse:Hsero_4280)

MetaCyc: 60% identical to phenylalanine hydroxylase (Chromobacterium violaceum)
Phenylalanine 4-monooxygenase. [EC: 1.14.16.1]

Predicted SEED Role

"Phenylalanine-4-hydroxylase (EC 1.14.16.1)" in subsystem Aromatic amino acid degradation or Pterin biosynthesis (EC 1.14.16.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.16.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IUL7 at UniProt or InterPro

Protein Sequence (295 amino acids)

>HSERO_RS21390 phenylalanine 4-monooxygenase (Herbaspirillum seropedicae SmR1)
MSTNPNTDDFFATVATKSDSGALRGDYSQADANYVVAQNWEGYTPAQHALWRRLYERQAR
LIPGRACDVFIESLKALDVSQGIPRFDRTTDALYKATGWQLVAVPGLVPDQTFFEHLANR
RFPVTVWLREEHEFDYIVEPDLFHDFFGHVPLLFNPVFANHLQEYGKGGLKALKLDGLAY
LARLYWYTIEFGLIQSEAGLRIYGAGILSSGGEVEYCLSSDKPRRVPFQVERIMRTLYKI
DTYQETYFVIRDFEQLFNDTAPDFTPFYERLKQQEPLPANALLAGETNLAANHLV