Protein Info for HSERO_RS20590 in Herbaspirillum seropedicae SmR1

Updated annotation (from data): fused 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase and oxepin-CoA hydrolase (EC 3.3.2.12; EC 1.2.1.91)
Rationale: Specific phenotype: utilization of Phenylacetic acid
Original annotation: enoyl-CoA hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 686 TIGR02278: phenylacetic acid degradation protein paaN" amino acids 8 to 682 (675 residues), 990.2 bits, see alignment E=2.1e-302 PF00171: Aldedh" amino acids 40 to 450 (411 residues), 198.2 bits, see alignment E=1.9e-62 PF01575: MaoC_dehydratas" amino acids 544 to 647 (104 residues), 106.3 bits, see alignment E=8.4e-35

Best Hits

KEGG orthology group: K02618, phenylacetic acid degradation protein (inferred from 100% identity to hse:Hsero_4121)

Predicted SEED Role

"Aldehyde dehydrogenase (EC 1.2.1.3), PaaZ" in subsystem Aromatic Amin Catabolism (EC 1.2.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.3

Use Curated BLAST to search for 1.2.1.3 or 1.2.1.91 or 3.3.2.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8ITG1 at UniProt or InterPro

Protein Sequence (686 amino acids)

>HSERO_RS20590 fused 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase and oxepin-CoA hydrolase (EC 3.3.2.12; EC 1.2.1.91) (Herbaspirillum seropedicae SmR1)
MTAHPPLLQSFIAGRWLGQRPSQALHSAVNGSAVASTHEESIDFGEALEHARKTGLPALM
ALDFQQRAAILKALAKYLMEHKEVLYAVSAHTGATRADSWIDIEGGSGTLFTYASIGANE
FPSSNLVHEGPAMRLGKQGRFAGTHILVPRGGVAVHINAFNFPVWGMLEKFAPTFLAGMP
CIVKPATATSYLAEAAVRLIQQSGLLPEGSLQLVIGSTGDLLERLQAQDVVTFTGSADTA
AKLRAHPNLIANSIPFNAEADSLNCAILGPDITADDEEFDLFVKEVAREMTTKAGQKCTA
IRRAIVPRRHVDAVAERLAARLSKIVVGDPALETVRMGPLASRAQQRDVEERVALLRHSA
DLILGAGSAFAPQGQGVGEGAFFAPTLLLARDTAAGSPVHEVEAFGPVSTLMPYDDLDEA
LALAARGRGSLVASLVTRTPAVAARAIPVAAAWHGRLLVLDREAAAESTGHGSPLPQLKH
GGPGRAGGGEELGGSRAVKHYLQRTAVQGSPTMLAAVVGEHVRGARVRESGIHPFRHYFE
DLQIGDSLTTHRRTVTEADIVNFGCLSGDHFYMHFDEIAARQSPFGKRIAHGYFVLSAAA
GLFVSPAPGPVLANYGLDTLRFVKPVGIGDTISARLTCKRKIDRNKKDANGQGQGVVAWD
VEVSNQEGELVASYDILTLVAKKPLE