Protein Info for HSERO_RS20550 in Herbaspirillum seropedicae SmR1

Annotation: acetyl-CoA carboxylase biotin carboxylase subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 5 to 113 (109 residues), 142.3 bits, E=1.7e-45
• PF02786: CPSase_L_D2
  • amino acids 118 to 325 (208 residues), 246.9 bits, E=3e-77
• PF07478: Dala_Dala_lig_C
  • amino acids 145 to 294 (150 residues), 30.7 bits, E=4.4e-11
• PF02785: Biotin_carb_C
  • amino acids 339 to 445 (107 residues), 123.9 bits, E=6.4e-40

Best Hits

Swiss-Prot: 51% identical to ACCC1_BACSU: Biotin carboxylase 1 (accC1) from Bacillus subtilis (strain 168)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 100% identity to hse:Hsero_4113)

MetaCyc: 50% identical to 4-hydroxyacetophenone carboxylase biotin carboxylase subunit (Aromatoleum aromaticum EbN1)
RXN-9833

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• 4-ethylphenol degradation (anaerobic) (2/6 steps found)
• jadomycin biosynthesis (3/9 steps found)
• 3-hydroxypropanoate cycle (5/13 steps found)
• glyoxylate assimilation (5/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (8/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (6/18 steps found)
• superpathway of mycolate biosynthesis (26/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Sequence Analysis Tools

See D8ITF3 at UniProt or InterPro

Protein Sequence (462 amino acids)

>HSERO_RS20550 acetyl-CoA carboxylase biotin carboxylase subunit (Herbaspirillum seropedicae SmR1)
MTTSLRKLLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARAD
RSYLNVDALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKA
EARRTAAAAGVPVVPGSAGELEDLATALACADEIGYPLLIKASAGGGGRGIRMARDATEL
AREFPLAQAEAQAAFGSAAVYLERFIRRARHIEVQILGDGERAVHLFERECSLQRRRQKV
LEEAPSPALTPAQRQALCDSAVRLAERLHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEH
PVTEMITGIDLVQAMLRIAGGEPLGLQQSDIRMQGAALEMRINAEDPERNFFPCPGTVAE
LQWPQGEGIRVESHLYAGYRIPPYYDSLLAKLVVHGKDRAQAIARAQAAVLATRITGMAT
TLSLHQWLLADARVQSARFDTGALETWLAERTDTRAKQLEEA