Protein Info for HSERO_RS20090 in Herbaspirillum seropedicae SmR1

Annotation: gluconolactonase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K01053, gluconolactonase [EC: 3.1.1.17] (inferred from 100% identity to hse:Hsero_4020)

MetaCyc: 50% identical to L-dehydro-ascorbate lactonase (Cupriavidus necator H16)
RXN-21400

Predicted SEED Role

"Gluconolactonase (EC 3.1.1.17)" in subsystem Entner-Doudoroff Pathway (EC 3.1.1.17)

MetaCyc Pathways

Entner-Doudoroff pathway III (semi-phosphorylative) (7/9 steps found)
sorbitol biosynthesis II (2/3 steps found)
Entner-Doudoroff pathway II (non-phosphorylative) (6/9 steps found)
glucose and glucose-1-phosphate degradation (3/5 steps found)
glucose degradation (oxidative) (3/5 steps found)
L-ascorbate biosynthesis IV (animals, D-glucuronate pathway) (3/6 steps found)
L-ascorbate biosynthesis VI (plants, myo-inositol pathway) (1/4 steps found)
L-ascorbate biosynthesis VIII (engineered pathway) (3/7 steps found)
L-ascorbate degradation V (1/5 steps found)
L-ascorbate degradation II (bacterial, aerobic) (2/7 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.17

Use Curated BLAST to search for 3.1.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8ISS5 at UniProt or InterPro

Protein Sequence (305 amino acids)

>HSERO_RS20090 gluconolactonase (Herbaspirillum seropedicae SmR1)
MSLPETPFQHFDARFRRMVIDTAQVDCLYTGCRWAEGPVWLPATQELIWSDIPNNRLMRW
SEGAGAGVFRQPSNFNNGNTLDREGRIVGCLHGGRAVVRTEHDGSITTLASHWQGKRLNS
PNDVVVKSDGSIWFTDPDYGINSDYEGYQAQSEIGACNVYRIDGDSGEISIVASDLERPN
GLAFSTDERRLYIADTGLTHRAGGPHHIRVFDVVDGISLKVGEVFATIDPGLADGFRLDA
QGNVWTSAGDGVHCYAPDGTLLGKILIPEVVSNVVFGGPRGNRLFITATTSLYAVYLAVN
GAQRP