Protein Info for HSERO_RS19775 in Herbaspirillum seropedicae SmR1

Annotation: acriflavine resistance protein B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1033 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 340 to 359 (20 residues), see Phobius details amino acids 366 to 386 (21 residues), see Phobius details amino acids 392 to 413 (22 residues), see Phobius details amino acids 437 to 459 (23 residues), see Phobius details amino acids 471 to 497 (27 residues), see Phobius details amino acids 540 to 561 (22 residues), see Phobius details amino acids 866 to 885 (20 residues), see Phobius details amino acids 892 to 912 (21 residues), see Phobius details amino acids 918 to 941 (24 residues), see Phobius details amino acids 966 to 987 (22 residues), see Phobius details amino acids 999 to 1024 (26 residues), see Phobius details PF00873: ACR_tran" amino acids 1 to 1021 (1021 residues), 1176.6 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1027 (1027 residues), 1428.8 bits, see alignment E=0 PF03176: MMPL" amino acids 336 to 499 (164 residues), 30.5 bits, see alignment E=1.9e-11

Best Hits

Swiss-Prot: 52% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 74% identity to azl:AZL_e02620)

MetaCyc: 52% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8ISL2 at UniProt or InterPro

Protein Sequence (1033 amino acids)

>HSERO_RS19775 acriflavine resistance protein B (Herbaspirillum seropedicae SmR1)
MPRFFIDRPIFAWVVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDG
VISLIERELSGVKNLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLPQ
TVRQNGVTVESAASSFLLMVSLTSVDGRYDEVRLNDYLARNIVEELRRMEGVGRVQLFGA
EQAMRIWLDPFKLNAYGLTVTDISTAISQQNAQIAPGRLGDAPAVRGQQIAMPLTVQGQL
QSPAQFAAIVLKAKADGSRVLLGDVARVELGAQSYAFANRENGKPAGVAAVQLSPGANAV
RTAAAIQARMEELRPSLPQGMAFSLPYNTAPFVKISIQKVVQTLIEAMVLVFLVMYLFLQ
NVRYTVIPAIVAPIALLGTLAVMQLAGYSVNVLTMFGMVLAIGIIVDDAIVVVENVERIM
AHEGLGPREATIKAMGEITGAIIGITLVLTAVFIPMALAGGSVGVIYRQFSVAMAVSILF
SAFLALSLTPALCATLLRPVPAGHHDKRGFFGAFNRAFNRLTARYESALGRLIARGLRMM
LVYAALSGLLVVGMQALPSAFLPEEDQGYFMTSFQLPADATTERTARAVDQFERYLKGRE
DIGANLSVLGFGFSGSGPNAAMAFTELKDWKERKGSTSAAEAMAAQAAMESGVDGMVMSL
LPPAIEELGNSSGFAMRLQDRANQGYAALKAAEAQLLALAAKSRKLSGVYSDGLPAGASV
QLEIDRHKAAALGVSFSSISDTLSAAMGSFYVNDFPNAGRMQQVILQAQASARMQLEDVM
KLYVRNQSGGMVPLAELVKASWREAPTQLQRYQGFPAVRISGSAAPGVASGEAMAEMERL
AQQLPPGFAVAWTGQSLQERLSAAQAPMLMALSMLVVFLVLAALYESWSIPLSVMLVVPL
GLVGAVAAVILRDMPNDVFFKVGMITVIGLSAKNAILIVEFARQLRSEGMGLVEAAIKAS
VLRLRPILMTSLAFALGVVPLMLASGASAETQRAIGTGVFGGMVSATVLAVLFVPVFFVL
VLGCAQRLARKGR