Protein Info for HSERO_RS19430 in Herbaspirillum seropedicae SmR1

Annotation: phosphoglycolate phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 226 PF00702: Hydrolase" amino acids 7 to 188 (182 residues), 109.5 bits, see alignment E=7.1e-35 PF12710: HAD" amino acids 10 to 185 (176 residues), 40.5 bits, see alignment E=1e-13 TIGR01449: phosphoglycolate phosphatase, bacterial" amino acids 11 to 219 (209 residues), 221.2 bits, see alignment E=2.3e-69 PF13419: HAD_2" amino acids 11 to 194 (184 residues), 123 bits, see alignment E=3.8e-39 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 84 to 191 (108 residues), 39.3 bits, see alignment E=1.4e-13 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 108 to 188 (81 residues), 38.3 bits, see alignment E=3.4e-13 PF13242: Hydrolase_like" amino acids 151 to 218 (68 residues), 36.4 bits, see alignment E=1e-12

Best Hits

Swiss-Prot: 47% identical to GPH_THIDA: Phosphoglycolate phosphatase (Tbd_2229) from Thiobacillus denitrificans (strain ATCC 25259)

KEGG orthology group: K01091, phosphoglycolate phosphatase [EC: 3.1.3.18] (inferred from 100% identity to hse:Hsero_3891)

Predicted SEED Role

"Phosphoglycolate phosphatase (EC 3.1.3.18)" in subsystem 2-phosphoglycolate salvage or Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 3.1.3.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.18

Use Curated BLAST to search for 3.1.3.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IS21 at UniProt or InterPro

Protein Sequence (226 amino acids)

>HSERO_RS19430 phosphoglycolate phosphatase (Herbaspirillum seropedicae SmR1)
MSKLTNIKAAIIDLDGTMLDTAADFHVAVNRMRDDLGLTPLSQETIVNFVGKGTENLIRR
VLAVDYAEDEAAQYFQQALAACTEHYLAINGDYSSLYPGVLEGLQALRNKGLRLACVTNK
PLAFAVPLLQKKGLRDFFEIVYGGDSFPKKKPDPMPLLQVCQDFALAPAQVVAIGDSSND
AQAARAAGCRVLNVPYGYNHGESIHDVDSDGIVSTLVEAAQQISVD