Protein Info for HSERO_RS18755 in Herbaspirillum seropedicae SmR1

Annotation: choline dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 543 PF00732: GMC_oxred_N" amino acids 135 to 324 (190 residues), 44.9 bits, see alignment E=1.9e-15 PF05199: GMC_oxred_C" amino acids 412 to 532 (121 residues), 72.1 bits, see alignment E=1.1e-23

Best Hits

Swiss-Prot: 50% identical to FDHL_GLUJA: Fructose dehydrogenase large subunit (fdhL) from Gluconobacter japonicus

KEGG orthology group: None (inferred from 100% identity to hse:Hsero_3758)

MetaCyc: 50% identical to fructose dehydrogenase large subunit (Gluconobacter japonicus)
FRUCTOSE-5-DEHYDROGENASE-RXN [EC: 1.1.5.14]

Predicted SEED Role

"2-Keto-D-gluconate dehydrogenase (EC 1.1.99.4), membrane-bound, flavoprotein" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.99.4)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.4

Use Curated BLAST to search for 1.1.5.14 or 1.1.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IRB0 at UniProt or InterPro

Protein Sequence (543 amino acids)

>HSERO_RS18755 choline dehydrogenase (Herbaspirillum seropedicae SmR1)
MNSNNKAEVVIVGTGVVGVLIAEQLLNAGIPVRMLEAGPRVARAEVVENFRNLPLALKGD
PSSCYPPRPWAPHPMPSSENPDDHYLELSGPDKYAQTFIRYAGGSTWHWAGTCWRLTPED
MRLHSLYGVGRDWAFGYDVLEPYYTRVEHQLGICGPSDPALQWPPQRSAPYPMPALPFSP
GEQRFTEVVQALGYRNIPTAQARNSGMPYDDRPACCANNNCVPVCPVGAKYDAATALARL
EAKGATILTDAVVYRVDTDANDRVEAVNYFDANKLSHRITGKIFVLACNGIETPKLLLMS
ANSRNPHGLANRSDQVGRNMMDHPQLTMTLTLSEPYWSGVGPVVNSGIMETSQGAFRSEH
AGAYFRYNNFARNRFVTFAALKKGLVGQALDEEIRRMTACTADIVLAHEVLPDAENRLTL
SSKKDWLGLPRPAIHYDVGDYTRRSAELYSLPIGRKIAEAMGATDIKVSPKFAQSKHIMG
GTIMGLDPANSVVDPDCRAHDHDNLYLPGGGAMPSTACGNSTISMAALGLKAADAILRQL
QKA