Protein Info for HSERO_RS18065 in Herbaspirillum seropedicae SmR1

Annotation: glutaminyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 583 PF00749: tRNA-synt_1c" amino acids 44 to 347 (304 residues), 354.5 bits, see alignment E=6.7e-110 TIGR00440: glutamine--tRNA ligase" amino acids 45 to 583 (539 residues), 691.7 bits, see alignment E=3.8e-212 PF03950: tRNA-synt_1c_C" amino acids 366 to 468 (103 residues), 78.3 bits, see alignment E=6.6e-26 PF20974: tRNA-synt_1c_C2" amino acids 488 to 560 (73 residues), 86.7 bits, see alignment E=2e-28

Best Hits

Swiss-Prot: 68% identical to SYQ_CUPMC: Glutamine--tRNA ligase (glnS) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

KEGG orthology group: K01886, glutaminyl-tRNA synthetase [EC: 6.1.1.18] (inferred from 100% identity to hse:Hsero_3620)

Predicted SEED Role

"Glutaminyl-tRNA synthetase (EC 6.1.1.18)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IQI6 at UniProt or InterPro

Protein Sequence (583 amino acids)

>HSERO_RS18065 glutaminyl-tRNA synthetase (Herbaspirillum seropedicae SmR1)
MSNELKNGHTPAPSTNFLRGIIESDLVSGVYAQRSNREGEALPQVVTRFPPEPNGYLHIG
HAKSICLNFGLAADYAGRCHMRFDDTNPEKEEQEYVDTILDSVKWLGFSWEHEGKSHLYY
ASNYFDVMYACAQALIRKGLAYVDEQNAEQMRENRGTLTEPGKNSPWRDRPAEESLRLFE
EMRDGKHPDGSMILRAKIDMASPNMNLRDPAIYRIRHATHHNTGDKWRVYPMYTFAHPIE
DALENISHSICTLEFEDQRPFYDWLLEQLAEEGFFKKPLPHQYEFARLNLTYIITSKRKL
RQLVDEKIVSGWDDPRMPTIVGLRRRGYTPEAIRLMCDRTGASKNNSWIDFSVLEGALRE
DLDPKAPRAVAVLRPLKLIIDNFPEGVSIDCSAPVYPPAHPEHDTAHRHFPITKELWIER
EDFMEEPTKGYFRLFPGNKVRLRYGYVVECTGCDKDEHGNVIAVHCNYFEDSKSGTEGSS
NYKVKGNLHWVSAEHALATEVRLYDRLFSDPHPDAGGKDFLAALNPNSKEVITAYLEPTL
KTAQPGEIYQFERHGYFVADRVDSTAGQPIFNRAVTLKDSWGK