Protein Info for HSERO_RS17625 in Herbaspirillum seropedicae SmR1

Annotation: tyramine oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 748 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF07833: Cu_amine_oxidN1" amino acids 28 to 100 (73 residues), 48.5 bits, see alignment E=1.7e-16 PF02727: Cu_amine_oxidN2" amino acids 115 to 200 (86 residues), 58.6 bits, see alignment E=1.2e-19 PF02728: Cu_amine_oxidN3" amino acids 207 to 308 (102 residues), 84.9 bits, see alignment E=8.4e-28 PF01179: Cu_amine_oxid" amino acids 329 to 742 (414 residues), 431.9 bits, see alignment E=5.1e-133

Best Hits

Swiss-Prot: 68% identical to AMO_ECOLI: Primary amine oxidase (tynA) from Escherichia coli (strain K12)

KEGG orthology group: K00276, primary-amine oxidase [EC: 1.4.3.21] (inferred from 100% identity to hse:Hsero_3531)

MetaCyc: 68% identical to copper-containing amine oxidase (Escherichia coli K-12 substr. MG1655)
Primary-amine oxidase. [EC: 1.4.3.21, 1.4.3.4]; 1.4.3.21 [EC: 1.4.3.21, 1.4.3.4]; 1.4.3.21 [EC: 1.4.3.21, 1.4.3.4]; 1.4.3.21 [EC: 1.4.3.21, 1.4.3.4]

Predicted SEED Role

"Monoamine oxidase (1.4.3.4)" in subsystem Aromatic Amin Catabolism or Auxin biosynthesis or Glycine and Serine Utilization or Threonine degradation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.21 or 1.4.3.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IQ98 at UniProt or InterPro

Protein Sequence (748 amino acids)

>HSERO_RS17625 tyramine oxidase (Herbaspirillum seropedicae SmR1)
LTTLTAAGLSTALLGPLPAAAHGAAAHMLPLKKTMEDFGATVRWDAYAGLWTIQRNSVTV
RVKPRATTALVNGQSVKLPVPLVMKGGQPYVSDEFINTVFAPSLDKTFQIEAVPHPLNPL
SADEINAALEVLKASGRWQPNYRFTEITLAEPPKEQVWKFALGERGQTIARRADFTLLDG
SKVIEGTVDLGQRNVTRWEPKEGVHGMVLVDDFATVQNAMESSPEYAQALAKRGITDIKK
VVATPLTVGYFGGKDALQEDARLLKVVSYLDVGDGNYWAHPIENLVAVVDLVQKKVIKIE
DNGVIPVPMKPTGYDGSGRALVPPPKPLDISEPEGKNYTITGNTLRWRNWEMHFKLDSRV
GLMFSTVTYNDHGKKRQIMYEGSLGGMIVPYGDPDVGWYFKAYLDSGEYGMGTLTSPIER
GKDAPDNVVLLDATLADTSGKPRTIPRAIGVFERYAGPEFKHQEYGQPNLSVERRELVIR
WISTVGNYDYIFDWVFAENGTIGINAGATGIEAVKGVKSSTMRDASAAEDTRYGTLIDHN
IVGTTHQHIYNFRLDLDVDGQNNSLTEVDPVVLPNTPGGPRSSTMQTVQRTAETEQQAAQ
KFDASTIRLLSNPNKFNKVGNPVSYQLIPYAGGTHPIAKGANFSPDEWLNKRLSFMDRQL
WVTRYDPQQRFPEGKYPNRSHEDTGLGAFVKDNQSIVNQDDVVWLTTGTTHVARAEEWPI
MPTEWVHVLLKPWNFFDETPTLGLNKKK