Protein Info for HSERO_RS15600 in Herbaspirillum seropedicae SmR1
Annotation: cytosol aminopeptidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 75% identical to AMPA_HERAR: Probable cytosol aminopeptidase (pepA) from Herminiimonas arsenicoxydans
KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 100% identity to hse:Hsero_3109)MetaCyc: 51% identical to aminopeptidase A/I (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; Leucyl aminopeptidase. [EC: 3.4.13.18, 3.4.11.1, 3.4.11.9]
Predicted SEED Role
"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)
MetaCyc Pathways
- γ-glutamyl cycle (5/6 steps found)
- glutathione degradation (DUG pathway) (2/2 steps found)
- muropeptide degradation (1/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.4.11.9
Use Curated BLAST to search for 3.4.11.1 or 3.4.11.9 or 3.4.13.18
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See D8J0N7 at UniProt or InterPro
Protein Sequence (506 amino acids)
>HSERO_RS15600 cytosol aminopeptidase (Herbaspirillum seropedicae SmR1) MDFSTKTLDAKNAVTAVKTGALVVGIYENRKLSKAAGALDKLGAISAALKSGDISGKPGS TLLLRGVTGIAAERIVLLGLGAEEEIADKSFATASSALVRTLATLGCDDAALALPFDGVK GRDLTWTIRNVVLAGRDAVYRSDTMKSKKDPAPTGVKKVTLLVPASDSVTAKAALAEAVA LANGMALTKELGNLPGNVCTPTYLANTAKKLAKQFGLGVEVLDRKQLQALKMGSFLSVTN GSDEPPKFIVLKHMGGKAKDAPVVLVGKGITFDTGGISLKPGLNMDEMKYDMCGAASVLG TFRTIAELGLKMNVIGVIPTCENMPSGRATKPGDIVTSMSGQTIEVLNTDAEGRLILCDA LTYVERFKPAAVVDIATLTGACIVALGHHNSGLFTREDEAHDALANALLAAGRDSGDTAW RMPIQEVYQEQLKSNFADIANIGGQPAGSVTAACFLERFTRKYTWAHLDIAGTAWKSGAA KGATGRPVPLLTTWLLNQARAKAARA