Protein Info for HSERO_RS15570 in Herbaspirillum seropedicae SmR1

Annotation: sulfate adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 23 to 223 (201 residues), 134.5 bits, E=5.2e-43
• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 23 to 440 (418 residues), 554.1 bits, E=1e-170
• PF03144: GTP_EFTU_D2
  • amino acids 271 to 330 (60 residues), 30.5 bits, E=6e-11

Best Hits

Swiss-Prot: 51% identical to CYSN_FRATN: Sulfate adenylyltransferase subunit 1 (cysN) from Francisella tularensis subsp. novicida (strain U112)

KEGG orthology group: K00956, sulfate adenylyltransferase subunit 1 [EC: 2.7.7.4] (inferred from 100% identity to hse:Hsero_3103)

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4)" in subsystem Cysteine Biosynthesis (EC 2.7.7.4)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (8/9 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• assimilatory sulfate reduction I (3/4 steps found)
• assimilatory sulfate reduction II (2/3 steps found)
• sulfate activation for sulfonation (1/2 steps found)
• assimilatory sulfate reduction IV (2/4 steps found)
• sulfite oxidation III (1/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (6/10 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (3/12 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.4

Use Curated BLAST to search for 2.7.7.4

Sequence Analysis Tools

See D8J0N1 at UniProt or InterPro

Protein Sequence (443 amino acids)

>HSERO_RS15570 sulfate adenylyltransferase (Herbaspirillum seropedicae SmR1)
MNAAATQAQLAQTAAPSHERGLLRFITAGSVDDGKSTLIGRLLFDSKGIFADQLDAISRA
KHKRTVGDTVDLSLLTDGLEAEREQGITIDVAYRYFATPKRKFIIADTPGHEQYTRNMVT
GASTADAVIILVDVSKVKLGEDGSVELLTQTKRHSTIAHLLQIQHVIVAVNKMDLVDYDQ
TVYDRIVGAYQQFAQQLGLQDVHAIPLSALAGDNVVEASARMPWYQGPTLITLLESLSVY
EDAHEEAFRFPVQLVARHNGHEANDFRGYMGRIEAGKVARGDKLVVLPSGQSATVKDIQT
LDGSLESASAGQSVTILLDEYLDISRGDLLASAAQPSTILKTVNADVCWLSEEPLDLRRK
YWLKHTTKQVAARVAKVDTLLDINTQERRPAETLKLNDIARISVNVQQPIAADAYQAIRA
TGAFILIDEVSHQTVAAGMIRID