Protein Info for HSERO_RS15225 in Herbaspirillum seropedicae SmR1

Annotation: 3-hydroxy-3-methylglutaryl-CoA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 TIGR00532: hydroxymethylglutaryl-CoA reductase, degradative" amino acids 12 to 394 (383 residues), 421.6 bits, see alignment E=1.8e-130 PF00368: HMG-CoA_red" amino acids 33 to 394 (362 residues), 373.2 bits, see alignment E=7.4e-116

Best Hits

Swiss-Prot: 69% identical to MVAA_PSEMV: 3-hydroxy-3-methylglutaryl-coenzyme A reductase (mvaA) from Pseudomonas mevalonii

KEGG orthology group: K00054, hydroxymethylglutaryl-CoA reductase [EC: 1.1.1.88] (inferred from 100% identity to hse:Hsero_3034)

MetaCyc: 72% identical to hydroxymethylglutaryl-CoA reductase (Delftia acidovorans SPH-1)
Hydroxymethylglutaryl-CoA reductase. [EC: 1.1.1.88]

Predicted SEED Role

"Hydroxymethylglutaryl-CoA reductase (EC 1.1.1.34)" in subsystem Archaeal lipids or Isoprenoid Biosynthesis or Leucine Degradation and HMG-CoA Metabolism (EC 1.1.1.34)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.34 or 1.1.1.88

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8J0G6 at UniProt or InterPro

Protein Sequence (436 amino acids)

>HSERO_RS15225 3-hydroxy-3-methylglutaryl-CoA reductase (Herbaspirillum seropedicae SmR1)
MSQDVHAGATPDSRLPGFRTYTPAQRLAEVGRLAQLDQAEQALLGQPGALPLGTADGMIE
NVVGSFQLPLGVAGYFQLNGRDVLVPMAVEEPSVVAAASFMAKLVRTCGGFQTSSSLPLM
RAQIQLTGISDPHGARLAILKERQAIIDIANSRDQLLQQLGGGCRDIEVQVFPDTRRGAM
VVTHLIVDVRDAMGANTVNTMAEAVASHIERVTGGKVRLRILSNLADLRLARARVKVSAD
VLSTAQYRGEEVINGIVDAYELAAVDPYRAATHNKGIMNGIDPVIVATGNDWRAVEAGAH
AYACRHGRYTSLTHWEIAADGDLVGTLEIPMPVGLVGGATKTHPAARVALKILGVQSAQA
LAEVAVAVGLAQNMAALRALATEGIQRGHMALHARNIALAAGAEASEMDWLVQQMVARHD
VRVDYAKALLAERRRG