Protein Info for HSERO_RS15005 in Herbaspirillum seropedicae SmR1

Annotation: ribosomal protein S12 methylthiotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 458 TIGR01125: ribosomal protein S12 methylthiotransferase RimO" amino acids 14 to 455 (442 residues), 560.9 bits, see alignment E=2.2e-172 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 14 to 455 (442 residues), 334.7 bits, see alignment E=7.8e-104 PF00919: UPF0004" amino acids 14 to 95 (82 residues), 69.5 bits, see alignment E=4.3e-23 PF04055: Radical_SAM" amino acids 154 to 336 (183 residues), 75.9 bits, see alignment E=8.9e-25 PF01938: TRAM" amino acids 390 to 453 (64 residues), 23 bits, see alignment E=1.2e-08 PF18693: TRAM_2" amino acids 392 to 456 (65 residues), 74.2 bits, see alignment E=1.5e-24

Best Hits

Swiss-Prot: 91% identical to RIMO_JANMA: Ribosomal protein S12 methylthiotransferase RimO (rimO) from Janthinobacterium sp. (strain Marseille)

KEGG orthology group: K14441, ribosomal protein S12 methylthiotransferase [EC: 2.-.-.-] (inferred from 100% identity to hse:Hsero_2992)

MetaCyc: 69% identical to ribosomal protein S12 methylthiotransferase RimO (Escherichia coli K-12 substr. MG1655)
RXN0-6366 [EC: 2.8.4.4]

Predicted SEED Role

"Ribosomal protein S12p Asp88 (E. coli) methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.- or 2.8.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8J0C4 at UniProt or InterPro

Protein Sequence (458 amino acids)

>HSERO_RS15005 ribosomal protein S12 methylthiotransferase (Herbaspirillum seropedicae SmR1)
MSNPSSPATPALPKVGFVSLGCPKALVDSEQILTQLRAEGYDTAKSYDGADLVIVNTCGF
IDAAVQESLDAIGEALNENGKVIVTGCLGAKKDAEGDDIIQKIHPKVLEVTGPHAVGEVM
QAVHKHLPKPHAPFIDLVPPQGVKLTPKHFAYLKISEGCNHRCSFCIIPSMRGDLVSRPI
ADVMLEAENLFKAGVKELLVISQDTSAYGVDVKFRTGFWNGKPVKTHMTQLVGALGELAQ
QYGAWVRLHYVYPYPHVDQIIPMMAEGKILPYLDVPLQHAHPDVLKRMKRPANGEKNIER
IQAWRAMCPDLTIRSTFIAGFPGETEAEFEYLLDFLKEAEIDRLGCFAYSPVEGATANEI
ANPVPEEVREERRGRVMQLQEEISKKRLQAKVGKTLRVLIDEVDRNGGTGRTYSDAPEID
GVVYVKPPFEPHRKLEVGQFVDVRITAADAHDLWGAAE