Protein Info for HSERO_RS14275 in Herbaspirillum seropedicae SmR1

Annotation: nitrogen fixation protein NifX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 133 TIGR02663: nitrogen fixation protein NifX" amino acids 1 to 119 (119 residues), 171.5 bits, see alignment E=4e-55 PF02579: Nitro_FeMo-Co" amino acids 11 to 104 (94 residues), 65.8 bits, see alignment E=1.8e-22

Best Hits

Swiss-Prot: 96% identical to NIFX_HERSE: Protein NifX (nifX) from Herbaspirillum seropedicae

KEGG orthology group: K02596, nitrogen fixation protein NifX (inferred from 100% identity to hse:Hsero_2848)

Predicted SEED Role

"Nitrogenase FeMo-cofactor carrier protein NifX" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IZK0 at UniProt or InterPro

Protein Sequence (133 amino acids)

>HSERO_RS14275 nitrogen fixation protein NifX (Herbaspirillum seropedicae SmR1)
MKVAFATQDLQRVDAHFGWAKNLAVFEVWPNGYSFVQTHSFDGDLKEDGDEDKLAPKIEA
IKDCAILYVAAIGGSGAARVVANRIHPVKVAQAEPILDILDKLQEVLKGTPAPWLRKAMQ
KGQERDINFEEEV