Protein Info for HSERO_RS14220 in Herbaspirillum seropedicae SmR1

Annotation: protein fixA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 281 PF01012: ETF" amino acids 23 to 212 (190 residues), 150.3 bits, see alignment E=2.7e-48

Best Hits

Swiss-Prot: 72% identical to FIXA_AZOVI: Protein FixA (fixA) from Azotobacter vinelandii

KEGG orthology group: K03521, electron transfer flavoprotein beta subunit (inferred from 100% identity to hse:Hsero_2837)

MetaCyc: 72% identical to quinone reductase (NADH,flavodoxin) complex electron transfer flavoprotein component alpha subunit (Azotobacter vinelandii)

Predicted SEED Role

"Electron transfer flavoprotein, beta subunit" in subsystem Acetyl-CoA fermentation to Butyrate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IYZ4 at UniProt or InterPro

Protein Sequence (281 amino acids)

>HSERO_RS14220 protein fixA (Herbaspirillum seropedicae SmR1)
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPAIINPYDLFSLEEALRIKDQVGGRVTVLT
MGPPQAEAALRKCISFGADDAILVTDRAFAGADTLATSYALASAIQRISQDQAVDLVLAG
KQTIDGDTAQVGPGIAKRLDLQLLTYVSKIVSLDPIAREIVIQRRAEGGVQVLQTRLPCL
ITMLEGSNEMRFATMENMFRAARHPIRTWDRTAAGIEDISKIGLKGSPTVVSKVFAPKPN
ARRAEIITAEGDEARDLAVTLVGKLFTQHPKLGEAIARKVA