Protein Info for HSERO_RS13620 in Herbaspirillum seropedicae SmR1

Annotation: diguanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1060 transmembrane" amino acids 13 to 35 (23 residues), see Phobius details amino acids 296 to 317 (22 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 329 to 451 (123 residues), 31.8 bits, see alignment E=1.4e-11 PF00989: PAS" amino acids 333 to 444 (112 residues), 23.7 bits, see alignment E=1.7e-08 PF08448: PAS_4" amino acids 341 to 449 (109 residues), 34.7 bits, see alignment E=7.4e-12 PF13426: PAS_9" amino acids 343 to 445 (103 residues), 26.2 bits, see alignment E=3.2e-09 PF01590: GAF" amino acids 470 to 611 (142 residues), 28.4 bits, see alignment E=8.6e-10 PF13185: GAF_2" amino acids 470 to 612 (143 residues), 35.5 bits, see alignment E=4.5e-12 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 621 to 783 (163 residues), 143.7 bits, see alignment E=4.3e-46 PF00990: GGDEF" amino acids 624 to 780 (157 residues), 169.1 bits, see alignment E=2.8e-53 PF00563: EAL" amino acids 801 to 1036 (236 residues), 262.5 bits, see alignment E=1.3e-81

Best Hits

KEGG orthology group: None (inferred from 100% identity to hse:Hsero_2714)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IYM5 at UniProt or InterPro

Protein Sequence (1060 amino acids)

>HSERO_RS13620 diguanylate cyclase (Herbaspirillum seropedicae SmR1)
MGKENKSPGRTGYTLKTLLLFVAFVGSFIVVQTWWEIRQDRQLTIDAERASSLTAARSIQ
EHATRVFNDLEQTMWSTAVQLHAGGPELLENDQGLYNVLASMQQRLPTVMVLRYVDRNGN
TRATSLRSLDTSSIPEDRKLTYVDGNPARPNLFVGQLMRSRYNRELVLPVAMNLYDRSAR
PIGLLVAELRAATFFDFYKRITNFEAIVSLRTESGSMMLRSPFEERWLDVNLADAKSMTY
IRAGGDEGSFEENSYLTGEPLLFAYRRLEKWSLVAVYARRMNDVLAPWRSRTENRILLTT
GILGFILIALISFLVYYRYQRRLDHALSASEYRYRKLYEEGNDPIVLISAELRYLDCNAA
ALRFFGVPDKERIIGRKVGLFSRQKSRHMEQPDIDELVARVLSGQPQQFEWVTVRRNKII
HTDVTLNRAEFERGYAIFAILRDISARKRAELLQSEQNRVLHMVMADDELDGVLYEIVNF
ADAQIPYGACSVMLVNEQASHFTSVLSHRYPPRLIRALQGMPIRHGCGVGAEAALRRGPC
IVANIAQDPITEHLRPLIDTEIYPSCGAWPIIGKEGQLLGVFAALLKENQAPSNEYLQLA
NIAADLASVAIESRRADERIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVIL
MDLDRFKNINDTFGHETGEAVLQEIAQRFRNSLRELDILARVGGDEFIVLVDDFDDPVQL
GEIAQKLLTEARKPFIIDGQEAMLSVSIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKD
DYRFFSDEINTNTVERIALEAELRRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPL
RGLLPPGEFITLAEETRLIDQIGLLVLDVACRDIGLMRQQQMTCGRISINLTGSQFGDER
LLDDIKEVVDRHGTPPSCLEFEITESMVMHNRERAIAIMDGIRAEGFTISIDDFGTGYSS
LAYLKRFPVNTLKIDKSFINDIPDNPNSSAIVQAIIAMSHALNLKVVTEGVETEKQLQAL
REFGSDEYQGYYFSKPVPYAQFVQLLAGQQDRPQGLARQG