Protein Info for HSERO_RS13270 in Herbaspirillum seropedicae SmR1

Annotation: bifunctional precorrin-3 methyltransferase/precorrin-6x reductase oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 519 PF00590: TP_methylase" amino acids 10 to 215 (206 residues), 128.5 bits, see alignment E=3.4e-41 TIGR01466: precorrin-3B C17-methyltransferase" amino acids 10 to 247 (238 residues), 288.3 bits, see alignment E=4.9e-90 TIGR00715: precorrin-6x reductase" amino acids 265 to 514 (250 residues), 195 bits, see alignment E=1.8e-61 PF02571: CbiJ" amino acids 266 to 511 (246 residues), 246.3 bits, see alignment E=3e-77

Best Hits

KEGG orthology group: None (inferred from 100% identity to hse:Hsero_2649)

Predicted SEED Role

"Cobalt-precorrin-3b C17-methyltransferase / Cobalt-precorrin-6x reductase (EC 1.3.1.54)" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis (EC 1.3.1.54)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.54

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IXP3 at UniProt or InterPro

Protein Sequence (519 amino acids)

>HSERO_RS13270 bifunctional precorrin-3 methyltransferase/precorrin-6x reductase oxidoreductase (Herbaspirillum seropedicae SmR1)
MEAVKKKGVLNLVSVGPGFEEMIIPRAIEALRESEVIVAYELYLRWIAAHIEGKEIHTPP
LTQERERALLAIEQARAGRRVALISSGDIGIYAMAALAFDDMREDDTYEVNVIPGVTSAN
ACASLLGSPLSHDFATLSLSNLLCPWTWIEQRASHIAQADLACVLYNVQSAGRQEGVYRI
LQLMLQHKAPHTLCGVVRNAYRPGQKVTIHRLDELPGLQFDMLTSIVIGNRFTQRKRDWI
FTPRGYNDWSEESQAEEVAQLPSQAVWVFAGTSDGNALAAELAGQGRPVVVSTATGYGGE
LARGDCPGAFVWSGRQGLEARRQALRDSRARVLVDATHPFAVQMSQQLMTLSQELGIPYL
RYERPGELAADDPHACADMEDAAHKAMAMGQRIFLATGSKDLATFLQAPGAQERQWFARV
TADPDFISRALELGLPRAHLCAMQGPFSQAFNEALWRDWGIDCVVTKDSGEAGGYHAKAE
AARALGIPLLTVRRPPLDYPQVAHDFGSVRAHLAALTGG