Protein Info for HSERO_RS13020 in Herbaspirillum seropedicae SmR1

Annotation: thymidylate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00041: dTMP kinase
  • amino acids 1 to 194 (194 residues), 177.6 bits, E=1.1e-56
• PF02223: Thymidylate_kin
  • amino acids 9 to 194 (186 residues), 169.3 bits, E=3.9e-54

Best Hits

Swiss-Prot: 81% identical to KTHY_JANMA: Thymidylate kinase (tmk) from Janthinobacterium sp. (strain Marseille)

KEGG orthology group: K00943, dTMP kinase [EC: 2.7.4.9] (inferred from 100% identity to hse:Hsero_2601)

Predicted SEED Role

"Thymidylate kinase (EC 2.7.4.9)" (EC 2.7.4.9)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotide phosphorylation (4/4 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)
• superpathway of pyrimidine deoxyribonucleoside salvage (5/9 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.9

Use Curated BLAST to search for 2.7.4.9

Sequence Analysis Tools

See D8IXJ5 at UniProt or InterPro

Protein Sequence (202 amino acids)

>HSERO_RS13020 thymidylate kinase (Herbaspirillum seropedicae SmR1)
MASGKFITFEGIDGAGKSTHIPFVTQLLRDRGLTVVATREPGGTELGERLRELVLHHKMH
LETEALLMFASRREHLAQVIEPALARGDWVISDRFTDATFAYQGGGRKLALDKLDRLEQW
VHPHLQPDLTLLFDVPLEVARARLDAERTLDKFEQEKADFFANTRAEYLRRAAQFPHRFR
LIDSTRPIAVIQEDLRAIVAAL