Protein Info for HSERO_RS12130 in Herbaspirillum seropedicae SmR1

Updated annotation (from data): 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22)
Rationale: Specifically important for utilizing m-Inositol.
Original annotation: 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR04377: 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase
  • amino acids 12 to 623 (612 residues), 937.9 bits, E=1.5e-286
• PF02776: TPP_enzyme_N
  • amino acids 14 to 139 (126 residues), 65.9 bits, E=4.5e-22
• PF00205: TPP_enzyme_M
  • amino acids 227 to 358 (132 residues), 120.4 bits, E=7.2e-39
• PF02775: TPP_enzyme_C
  • amino acids 426 to 579 (154 residues), 118.6 bits, E=3.2e-38

Best Hits

KEGG orthology group: K03336, 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase [EC: 3.7.1.-] (inferred from 100% identity to hse:Hsero_2428)

Predicted SEED Role

"Epi-inositol hydrolase (EC 3.7.1.-)" in subsystem Inositol catabolism (EC 3.7.1.-)

MetaCyc Pathways

• myo-inositol degradation I (7/7 steps found)
• myo-, chiro- and scyllo-inositol degradation (7/10 steps found)

KEGG Metabolic Maps

• 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
• Ascorbate and aldarate metabolism
• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Biphenyl degradation
• Caprolactam degradation
• Ethylbenzene degradation
• Inositol phosphate metabolism
• Limonene and pinene degradation
• Nicotinate and nicotinamide metabolism
• Phenylalanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.7.1.- or 3.7.1.22

Sequence Analysis Tools

See D8IVI8 at UniProt or InterPro

Protein Sequence (625 amino acids)

>HSERO_RS12130 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22) (Herbaspirillum seropedicae SmR1)
MNAQVNVRPTLRLTMAQALVRYLDALRIQTPQGVLPLFGGVFSIFGHGNVAGMGEALYQY
RATLPTYRAHNEQAMAHSAIAYAKAHLRQRMMAVTTSIGPGATNLLTAAALAHVNRLPVL
LLPGDVFVSRRPDPVLQQLEDFNDPGLSVNDAFRPLSRMFDRICYPEQLLTALPRAIQVL
TDPAQCGPVTLALPQDVQTMAYDYPLDFFSPRVIVPRAQAPAAQELEDAVALLRQARQPL
LIAGGGVLYGNACEHLRRFAEQHGVPVAETQAGKSALPWSHALQMGAIGVTGSPAANALA
QEADVVIAVGTRLQDFTTGSHTLFAQARLLNLNVNAMDAHKWRGLALQADAGLGLEALSH
ALEGWRSAPEWHARAHTLAQDWRERVAQITGQTDTGGRLPYDGEVIGAIQRSVADSPSQD
IVVCAAGTLPAELHKLWRAGRPGAYHVEYGYSCMGYEVAGGLGVKLAQPQREVIVIVGDG
SYLMMNSELATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNMLADCLQAGPGAPA
IDFAAHARSLGALGENVKTITELEAALQRARAADRSYLVCIDTDASRTTDDGGCWWEVAV
PEVSPRSQVQQARSQYEQARQAQSV