Protein Info for HSERO_RS12045 in Herbaspirillum seropedicae SmR1

Annotation: trans-aconitate 2-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 267 PF13489: Methyltransf_23" amino acids 25 to 145 (121 residues), 56.7 bits, see alignment E=7.4e-19 PF05175: MTS" amino acids 33 to 110 (78 residues), 38.3 bits, see alignment E=3.3e-13 PF13847: Methyltransf_31" amino acids 43 to 151 (109 residues), 54.4 bits, see alignment E=3.8e-18 PF13649: Methyltransf_25" amino acids 45 to 134 (90 residues), 63.2 bits, see alignment E=9.3e-21 PF08242: Methyltransf_12" amino acids 46 to 136 (91 residues), 49 bits, see alignment E=2.5e-16 PF08241: Methyltransf_11" amino acids 46 to 137 (92 residues), 53.7 bits, see alignment E=8.4e-18

Best Hits

Swiss-Prot: 44% identical to TAM_VARPS: Trans-aconitate 2-methyltransferase (tam) from Variovorax paradoxus (strain S110)

KEGG orthology group: K00598, trans-aconitate 2-methyltransferase [EC: 2.1.1.144] (inferred from 100% identity to hse:Hsero_2410)

Predicted SEED Role

"Trans-aconitate 2-methyltransferase (EC 2.1.1.144)" (EC 2.1.1.144)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.144

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IVH0 at UniProt or InterPro

Protein Sequence (267 amino acids)

>HSERO_RS12045 trans-aconitate 2-methyltransferase (Herbaspirillum seropedicae SmR1)
MQDSQDIMQPQVQWDVSQYLRFGGERLRPALDLMLHLPTQTPARILDLGCGAGNLSAILQ
QRWPTAEVLGVDGSEDMLAKARMNVPQGRFIAADLNHWTPAHKLDLIYTNATLQWLPDHA
QLFPRLLSFLNPGGCLAVQMPVMHDAPLRRAQVELARSPAYADVLGEVSERRNVLEMGGY
YDLLRPRVASLDIWQTTYLHILTGADPVLEWASGSSLRPYLEKLAGEQLQAFRADYARRM
QLAYPPSADGSTLLPFQRMFIVASLPG