Protein Info for HSERO_RS10665 in Herbaspirillum seropedicae SmR1

Annotation: coproporphyrinogen III oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 414 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR00539: putative oxygen-independent coproporphyrinogen III oxidase" amino acids 41 to 381 (341 residues), 364.7 bits, see alignment E=2.7e-113 PF04055: Radical_SAM" amino acids 44 to 211 (168 residues), 81.9 bits, see alignment E=6.3e-27 PF06969: HemN_C" amino acids 342 to 403 (62 residues), 42.8 bits, see alignment E=4.3e-15

Best Hits

Swiss-Prot: 50% identical to HEMW_HAEIN: Heme chaperone HemW (hemW) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K02495, oxygen-independent coproporphyrinogen III oxidase [EC: 1.3.99.22] (inferred from 100% identity to hse:Hsero_2135)

Predicted SEED Role

"Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB" in subsystem Heat shock dnaK gene cluster extended or Heme and Siroheme Biosynthesis or Queuosine-Archaeosine Biosynthesis

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.22

Use Curated BLAST to search for 1.3.99.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IT76 at UniProt or InterPro

Protein Sequence (414 amino acids)

>HSERO_RS10665 coproporphyrinogen III oxidase (Herbaspirillum seropedicae SmR1)
MISIKPVPVTAVAGQGASGPAQAALAFLKPGSLQLTALPPLSLYVHFPWCVRKCPYCDFN
SHEVKDGFPEQEYLDALRLDLEQALPLIWGRKIYSIFIGGGTPSLLSAAGLDRLLSDLRT
LLPLDADIEITMEANPGTFEADKFRSYRDSGINRLSIGIQSFNSAHLQALGRIHDGGQAH
QAVEIAHANFDNFNLDLMYALPQQTLEQARTDIQTAIDFAPPHLSLYHLTLEPNTLFAKF
PPAVPDEDTSADMQDMIGELTTQAGYGNYEVSAYAQPGRRARHNLNYWQFGDYLGIGAGA
HSKLSFPHRIVRQMRYKHPQAYLQHIKAGTHIQEEAEIGRDALGFEFMLNALRLTEGFPP
NLFAERTGLALNQIGKQLDLAERKGMLYRDHALIRPTPLGRRFLNDLQEIFLSG