Protein Info for HSERO_RS10600 in Herbaspirillum seropedicae SmR1

Annotation: diguanylate phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 transmembrane" amino acids 331 to 349 (19 residues), see Phobius details PF00563: EAL" amino acids 78 to 205 (128 residues), 38.9 bits, see alignment E=6.7e-14 PF08668: HDOD" amino acids 229 to 341 (113 residues), 46.7 bits, see alignment E=2.8e-16

Best Hits

KEGG orthology group: K07181, putative signal transduction protein containing EAL and modified HD-GYP domains (inferred from 100% identity to hse:Hsero_2121)

Predicted SEED Role

"Predicted signal transduction protein" in subsystem Flagellar motility

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IT62 at UniProt or InterPro

Protein Sequence (448 amino acids)

>HSERO_RS10600 diguanylate phosphodiesterase (Herbaspirillum seropedicae SmR1)
MSDQSSDQSTSTHQALDFYLARQPILSREQHLIGYELLFRSAAFGPANVKDDVTATAAVI
AHASELGLSNVIGNLQGFINVDAAVLMSDFIYFLPNDKVVLEILETVRVTPELVVRVREL
VKAGYVFALDDVVAESQEIEELLPLVKIIKIDVMEVDPSKLLKLSVHFKRAQKELLAEKV
ETLQQFNDCVTFGFDYFQGYYFAKPMILQGKKLEASKMVIMHLLTLLVRNVDNGEIVRFI
KRDVALSLTLLRLVNTPVYGFQKFIDSLGQALIVLGRRQLKRWLQILLFANTDKEQAHTM
SPLMILAATRGKMLELITTRIRPGRRKMSEMAFTVGIMSLVDALFGMSMETVLRQITVST
EIREALLRRTGFYGVALRLVECTEQRDQDQAEMQRLLDELQLSADDFFEAEKHAFEWGNS
ISNNNIGAQASMHMSPPPEGFIDDDDDD