Protein Info for HSERO_RS10045 in Herbaspirillum seropedicae SmR1

Annotation: UDP-glucose 4-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08659: KR
  • amino acids 4 to 134 (131 residues), 30.8 bits, E=9.4e-11
• PF00106: adh_short
  • amino acids 5 to 88 (84 residues), 33.5 bits, E=1e-11
• PF04321: RmlD_sub_bind
  • amino acids 5 to 162 (158 residues), 45 bits, E=2.6e-15
• TIGR01179: UDP-glucose 4-epimerase GalE
  • amino acids 6 to 333 (328 residues), 457.6 bits, E=9.6e-142
• PF01370: Epimerase
  • amino acids 6 to 264 (259 residues), 197.1 bits, E=1.1e-61
• PF02719: Polysacc_synt_2
  • amino acids 6 to 185 (180 residues), 58.2 bits, E=2.8e-19
• PF01073: 3Beta_HSD
  • amino acids 7 to 161 (155 residues), 61.2 bits, E=3e-20
• PF16363: GDP_Man_Dehyd
  • amino acids 7 to 327 (321 residues), 194.7 bits, E=1e-60

Best Hits

Swiss-Prot: 65% identical to GALE_BACSU: UDP-glucose 4-epimerase (galE) from Bacillus subtilis (strain 168)

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 100% identity to hse:Hsero_2010)

MetaCyc: 65% identical to UDP-glucose 4-epimerase (Bacillus subtilis subtilis 168)
UDP-N-acetylglucosamine 4-epimerase. [EC: 5.1.3.7]; UDP-glucose 4-epimerase. [EC: 5.1.3.7, 5.1.3.2]

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (9/11 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• D-galactose detoxification (1/3 steps found)
• stachyose degradation (2/7 steps found)
• UDP-sugars interconversion (2/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2 or 5.1.3.7

Sequence Analysis Tools

See D8ISG7 at UniProt or InterPro

Protein Sequence (343 amino acids)

>HSERO_RS10045 UDP-glucose 4-epimerase (Herbaspirillum seropedicae SmR1)
MTQATILVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVIDRIAQISGQRPHFVQG
DIRDRAVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEAMAAHGVK
NIVFSSSATVYGDPASVPITEEFPLSATNPYGRSKLMVEQILGDLHVADPSWRIALLRYF
NPVGAHESGLIGEDPSGIPNNLLPFIAQVADGRRAALSVYGSDYPTPDGTGVRDYIHVVD
LALGHLKTLRKLEQGPGVYTYNLGTGRGNSVLEMLAAFEQACGNKLPYQLVDRRPGDIAC
CYAATERAERELGWRAQRGIEAMCADTWRWQALAKRLAQEEAA