Protein Info for HSERO_RS10000 in Herbaspirillum seropedicae SmR1

Annotation: mannose-1-phosphate guanylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 471 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase" amino acids 3 to 470 (468 residues), 708.6 bits, see alignment E=1.8e-217 PF00483: NTP_transferase" amino acids 5 to 288 (284 residues), 202.5 bits, see alignment E=2.2e-63 PF12804: NTP_transf_3" amino acids 6 to 140 (135 residues), 36.3 bits, see alignment E=1.6e-12 PF22640: ManC_GMP_beta-helix" amino acids 302 to 348 (47 residues), 61.2 bits, see alignment 1.8e-20 PF01050: MannoseP_isomer" amino acids 352 to 466 (115 residues), 192 bits, see alignment E=7.2e-61 PF07883: Cupin_2" amino acids 383 to 450 (68 residues), 41.4 bits, see alignment E=2.4e-14

Best Hits

Swiss-Prot: 59% identical to MANC1_ECO57: Mannose-1-phosphate guanylyltransferase 1 (manC1) from Escherichia coli O157:H7

KEGG orthology group: None (inferred from 100% identity to hse:Hsero_2001)

MetaCyc: 59% identical to mannose-1-phosphate guanylyltransferase (Escherichia coli K-12 substr. MG1655)
Mannose-1-phosphate guanylyltransferase. [EC: 2.7.7.13]

Predicted SEED Role

"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.13 or 2.7.7.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8ISF8 at UniProt or InterPro

Protein Sequence (471 amino acids)

>HSERO_RS10000 mannose-1-phosphate guanylyltransferase (Herbaspirillum seropedicae SmR1)
MKIYPVILSGGAGTRLWPLSRAAYPKQLLPLVSEQTMLQETVARVCAWPEALAPLVVCGN
EHRFMIAEQLREMDVRPHAIVLEPQGKNTAPAVAAAALQLMRQDPDAVMLVLPADHVITN
REAFHEAVRAAMRSVEQGALVTFGITPSKPETGYGYILRGAAHGDTGNFAVERFVEKPDL
ERAQGFVADGRYYWNSGMFLLRARDYLEELELHRPLIAAAVKDAVAKAYTDLDFCRLGEA
AFGASPAESIDYAVMENTRRAVVVPADIGWSDVGSWSALQEVMPADAQGNVTRGDVYLDG
VSNSLVRAESRMVAVLGLQDLIVVETDDAVLVAHKDKAQDVKGVVDQLKDKKRSEHIHHK
RVYRPWGSYESVDAGDRFQVKRIIVKPGEKLSLQMHYHRAEHWVVVSGSALVTRGEEVTL
LSENESIYLPIGVTHRLENPGKLPLHLIEVQSGSYLGEDDIVRFDDVYKRA