Protein Info for HSERO_RS09900 in Herbaspirillum seropedicae SmR1

Annotation: phenylalanyl-tRNA synthetase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 342 PF02912: Phe_tRNA-synt_N" amino acids 24 to 91 (68 residues), 80.6 bits, see alignment E=6.7e-27 TIGR00468: phenylalanine--tRNA ligase, alpha subunit" amino acids 42 to 341 (300 residues), 327.2 bits, see alignment E=5.6e-102 PF01409: tRNA-synt_2d" amino acids 96 to 341 (246 residues), 314.4 bits, see alignment E=5.4e-98

Best Hits

Swiss-Prot: 92% identical to SYFA_JANMA: Phenylalanine--tRNA ligase alpha subunit (pheS) from Janthinobacterium sp. (strain Marseille)

KEGG orthology group: K01889, phenylalanyl-tRNA synthetase alpha chain [EC: 6.1.1.20] (inferred from 100% identity to hse:Hsero_1981)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8ISD8 at UniProt or InterPro

Protein Sequence (342 amino acids)

>HSERO_RS09900 phenylalanyl-tRNA synthetase subunit alpha (Herbaspirillum seropedicae SmR1)
MNPLDQLLGQLVTQAQADFAAAPDAAALENAKAKYLGKTGQITEQMKGLGKLAPEERKVQ
GAIINVAKEQIETALTARRDALANAQMQARLNAEAIDVTLPGRGRGVGGIHPVMRSWQRV
EEIFRSIGFDVADGPEIETDWTNFTALNSPENHPARSMQDTFYIEGNDSQGKPLLLRTHT
SPMQVRYARMNKPPIKVIAPGRTYRVDSDATHSPMFHQVEGLWIAEDISFADLKGVYLNF
VKAFFETDDLQVRFRPSYFPFTEPSAEIDIAFGSGPLKGRWLEVSGAGQVHPTVVRNMGL
DPEQYIGFAFGSGLERLTMLRYGINDLRLFYEGDLRFLKQFN