Protein Info for HSERO_RS09855 in Herbaspirillum seropedicae SmR1

Annotation: GTP pyrophosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 749 PF13328: HD_4" amino acids 30 to 197 (168 residues), 138.7 bits, see alignment E=3.6e-44 TIGR00691: RelA/SpoT family protein" amino acids 45 to 748 (704 residues), 597.8 bits, see alignment E=1.4e-183 PF04607: RelA_SpoT" amino acids 256 to 365 (110 residues), 150.4 bits, see alignment E=5.6e-48 PF02824: TGS" amino acids 419 to 478 (60 residues), 76.8 bits, see alignment 2.7e-25 PF19296: RelA_AH_RIS" amino acids 497 to 653 (157 residues), 24.5 bits, see alignment E=6.6e-09 PF13291: ACT_4" amino acids 673 to 748 (76 residues), 55.4 bits, see alignment E=1.9e-18

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 100% identity to hse:Hsero_1973)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8ISD2 at UniProt or InterPro

Protein Sequence (749 amino acids)

>HSERO_RS09855 GTP pyrophosphokinase (Herbaspirillum seropedicae SmR1)
MVSVASTQGEGLAALTAGLGPEDAARVAQALAELEPLYADRQILSGQNALQFSISVATVL
TALNVDAATRIAALAFEAQMLHPELEEKLEERFGKEIADLVGNVRQLMRFHSFSFQPQQE
VLRGKNAAQQAAAQIETLRKMLLAMATDMRAVLVRLASRVATLRYFAETKQEDEASANYA
RQTFELYAPLANRLGIWQLKWELEDLSFRFLEPVTYKRIAKMLEEKRLEREAFVANAIAR
LRAEMAAQDIRAEVSGRPKHIYSIWNKMRGKHIDFSELYDVRAFRVIVDDVKTCYAVLGI
VHDIWTPIPEEFDDYISRPKQNGYQSLHTVVTAEDGRALEVQIRTHEMHHFAEYGVAAHW
RYKESGGSNFSAQKYDEKIAWLRQLLAWKNEVTDAVVDQEEVRRDWVEKLKSATLDERIY
VLTPQARVIELPSGATPIDFAYHLHSDVGHRCRGARVDGVMVPLNTALKNGQTVEIITVK
SGPGVGPSRDWLSPDYAVSSRTRSKIRAWFNAIEQQETLSVGRGLLEKTLQREGKTAVNL
EELAQKLGFAKVDDLCLSLAKEEFSLRHVEQALHEGQEKKPEIDDETLITRKSRASSIVQ
GAKSGVLVVGTDGLLTQLARCCKPAPPDAIAGFVTRGKGVSIHRVSCKNFAEMSSHAPER
VIQTTWGAPARETVYPVDIFVLASDRQGLLRDISDIFLRDKINVIGVSTQSVKGHARMAF
TAEIGSTEQLQKALTVIREVKGVIEAKRQ