Protein Info for HSERO_RS08875 in Herbaspirillum seropedicae SmR1

Annotation: NADH:ubiquinone oxidoreductase subunit L

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 696 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 44 to 64 (21 residues), see Phobius details amino acids 92 to 113 (22 residues), see Phobius details amino acids 125 to 142 (18 residues), see Phobius details amino acids 148 to 167 (20 residues), see Phobius details amino acids 187 to 206 (20 residues), see Phobius details amino acids 226 to 242 (17 residues), see Phobius details amino acids 263 to 281 (19 residues), see Phobius details amino acids 293 to 313 (21 residues), see Phobius details amino acids 321 to 345 (25 residues), see Phobius details amino acids 350 to 370 (21 residues), see Phobius details amino acids 388 to 409 (22 residues), see Phobius details amino acids 429 to 450 (22 residues), see Phobius details amino acids 509 to 531 (23 residues), see Phobius details amino acids 566 to 590 (25 residues), see Phobius details amino acids 670 to 691 (22 residues), see Phobius details TIGR01974: proton-translocating NADH-quinone oxidoreductase, chain L" amino acids 9 to 690 (682 residues), 801.8 bits, see alignment E=3e-245 PF00662: Proton_antipo_N" amino acids 76 to 126 (51 residues), 81.3 bits, see alignment 3.7e-27 PF00361: Proton_antipo_M" amino acids 142 to 425 (284 residues), 270.7 bits, see alignment E=1.5e-84

Best Hits

Swiss-Prot: 63% identical to NUOL_NEIMB: NADH-quinone oxidoreductase subunit L (nuoL) from Neisseria meningitidis serogroup B (strain MC58)

KEGG orthology group: K00341, NADH dehydrogenase I subunit L [EC: 1.6.5.3] (inferred from 100% identity to hse:Hsero_1778)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IRG6 at UniProt or InterPro

Protein Sequence (696 amino acids)

>HSERO_RS08875 NADH:ubiquinone oxidoreductase subunit L (Herbaspirillum seropedicae SmR1)
MAGQLNPHLLLAVPLAPLAGSAIAGLLGTKFFGNVVGRKVSHTATILGVLIAAIISLQTL
LAVLDGATYNGTLYNWMTVAGLKLEIGFMVDSLTAMMMCVVTFVSLMVHIYTIGYMAEDE
GYNRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVSYLLIGFWYTRPTAIKANMKAF
LVNRVGDFGFILGIGLLLAYAGSMNYTEVFDKRAELAQLTLPGTDWMLLTVACICLFVGA
MGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVTRMSPLFELSDTALSFILVI
GAITALFMGFLGTMQNDIKRVVAYSTLSQLGYMTVALGASAYSVAVFHLMTHAFFKALLF
LGAGSVIIGMHHDQDMRNMGGLRKYMPITWITSLLGSLALIGTPFFSGFYSKDSIIEAAA
ESHLAGSGFAYFAVLASVFVTAFYSFRMYFMVFHGKERFGQAHGHDDHHAPAAAHGVHDD
HAHDAHDDHGHDEHHHGLAPGQKPHESPLVVTLPLILLAIPSVIIGFFAISPMLYGDFFK
GVIFFGENHKVMEELAHEYHGPVAMVLHAFTSAPLWLAIAGVVVAYYCYMINPRVPAWFK
EKFSIIYTILDNKYYMDKFNEVVFAGGARLLGSGLSTVGDRGLIDGLFVNGSAKVVGWFS
KFTRLWQSGYIYHYAFVMIIGVLGFLVWFMPFPFAK