Protein Info for HSERO_RS08480 in Herbaspirillum seropedicae SmR1

Annotation: chemotaxis protein CheA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 837 transmembrane" amino acids 806 to 831 (26 residues), see Phobius details PF01627: Hpt" amino acids 4 to 92 (89 residues), 41.4 bits, see alignment E=2.7e-14 PF02895: H-kinase_dim" amino acids 449 to 516 (68 residues), 62.9 bits, see alignment E=6.1e-21 PF02518: HATPase_c" amino acids 564 to 703 (140 residues), 61.2 bits, see alignment E=2.5e-20 PF01584: CheW" amino acids 709 to 836 (128 residues), 58.4 bits, see alignment E=1.2e-19

Best Hits

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 100% identity to hse:Hsero_1704)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IQV6 at UniProt or InterPro

Protein Sequence (837 amino acids)

>HSERO_RS08480 chemotaxis protein CheA (Herbaspirillum seropedicae SmR1)
MTLLRDQFILETRDLLQGISEKLMQLEDAPEHVELLESLFRMVHTLKGNSGLFDVPEMTR
VLHACEDLMDAVRRRQVAYSEVLADRLLDAMDFVAMLCDGLEMEAEQADPHAQEAVQIAS
SLRQLIGEHAGVESAVGDLARIGDAQDRGAAIVAAPAAGQALPLRAIPEPLRRQAFAQAQ
AGGELHWIAYRPSRECFFQGDDPFHRAREVPGFIWGRIEAPAAWPPLAELNAYDCVLHFQ
IVAAASQAELMEYFRYVPDQVELTQLAAQWLAPAPESAERASAGAHAEPAQDPIDAVMAE
IFAIQRQILQSEANMGWQDGRTQAVASTLMNCCRSIGNLELIGELEIARQQSLQLRSCDP
LLAWLNANPLPLQRGPVASVASVASAEAGAEAAEAVAVEVRQESLPQPEALSRQEPPQVQ
PPAPPLRESLPEESGRAGRRAEESLSGNKSVKVEQVKIDRLMNLIGEMVVSKNALPYLAQ
RAEDQFGVRELAREIKAQYAVINRIAEEMQDAIMQVRMMPVSVVFQRFPRLVRDISRKLG
KEVQLVLVGEETEADKNIIEALADPLIHIVRNSLDHGLEGPEQRRASGKPALGSLTISAA
HEGDQVVIVIEDDGKGIDPALIKRKAYEKGLIDEAQLERISDQDAVNLVFSSGFSTAEVV
SDLSGRGVGMDVVRNAVEKVNGSLALHSVSGKGTRLQISLPLSMAVTKVMIIESDGQLFG
VPMEHVMETVRVPRSSLHFANSSAKMVLRGHILGLKGINQILGIAAEQKTNADGEVAVLV
VRVGGQSVGLVIDDFRETVDVIQKPLLGILGGLSLYSGSALMGDGSVLMVLNTREMF