Protein Info for HSERO_RS08170 in Herbaspirillum seropedicae SmR1

Annotation: S-adenosyl-L-methionine (SAM)-dependent methyltransferase PhcB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 253 PF01209: Ubie_methyltran" amino acids 35 to 151 (117 residues), 64.1 bits, see alignment E=5.1e-21 PF07021: MetW" amino acids 35 to 136 (102 residues), 23.2 bits, see alignment E=2e-08 PF13489: Methyltransf_23" amino acids 40 to 189 (150 residues), 63.4 bits, see alignment E=8.5e-21 PF03141: Methyltransf_29" amino acids 42 to 145 (104 residues), 34.9 bits, see alignment E=2.8e-12 PF13847: Methyltransf_31" amino acids 44 to 155 (112 residues), 74.9 bits, see alignment E=2.4e-24 PF13649: Methyltransf_25" amino acids 46 to 139 (94 residues), 80.3 bits, see alignment E=5.7e-26 PF08241: Methyltransf_11" amino acids 47 to 143 (97 residues), 86 bits, see alignment E=9.1e-28 PF08242: Methyltransf_12" amino acids 47 to 141 (95 residues), 56.1 bits, see alignment E=2.1e-18

Best Hits

Swiss-Prot: 50% identical to YAFE_ECOLI: Uncharacterized protein YafE (yafE) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to hse:Hsero_1643)

Predicted SEED Role

"SAM-dependent methyltransferase YafE (UbiE paralog)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IQP5 at UniProt or InterPro

Protein Sequence (253 amino acids)

>HSERO_RS08170 S-adenosyl-L-methionine (SAM)-dependent methyltransferase PhcB (Herbaspirillum seropedicae SmR1)
MRSHQQVVTEQFGSTAAAYLSSTVHAQGADLQELAAIAAAIPAARVLDLGCGGGHASFAV
APVVEKVIAYDLSPAMLDVVATAAAERGLANLAVRQGSADRLDFPDASFDLVCTRFSAHH
WRNLPQALAEVFRVLKMGGRFIVIDTAAPADVLADTYVQSIELLRDTSHVRNISLRSWRK
LLARAGFGVSSDKTWKLRLEFDSWVARMRTPQQNIVAIKALWQAAPEEVRQYFALDEAGS
FSIDVAMLDACKL