Protein Info for HSERO_RS08020 in Herbaspirillum seropedicae SmR1

Annotation: heavy metal translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 744 transmembrane" amino acids 92 to 112 (21 residues), see Phobius details amino acids 123 to 141 (19 residues), see Phobius details amino acids 155 to 175 (21 residues), see Phobius details amino acids 187 to 207 (21 residues), see Phobius details amino acids 342 to 364 (23 residues), see Phobius details amino acids 373 to 397 (25 residues), see Phobius details amino acids 689 to 707 (19 residues), see Phobius details amino acids 713 to 731 (19 residues), see Phobius details PF00403: HMA" amino acids 13 to 72 (60 residues), 65.8 bits, see alignment 5.6e-22 TIGR01511: copper-translocating P-type ATPase" amino acids 137 to 732 (596 residues), 585.6 bits, see alignment E=2.3e-179 TIGR01525: heavy metal translocating P-type ATPase" amino acids 155 to 731 (577 residues), 632 bits, see alignment E=2.2e-193 TIGR01494: HAD ATPase, P-type, family IC" amino acids 198 to 709 (512 residues), 284.3 bits, see alignment E=3e-88 PF00122: E1-E2_ATPase" amino acids 226 to 405 (180 residues), 201.8 bits, see alignment E=9.9e-64 PF00702: Hydrolase" amino acids 422 to 643 (222 residues), 122.5 bits, see alignment E=4.4e-39

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 100% identity to hse:Hsero_1608)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IQ75 at UniProt or InterPro

Protein Sequence (744 amino acids)

>HSERO_RS08020 heavy metal translocating P-type ATPase (Herbaspirillum seropedicae SmR1)
MNDSTATRTDISLDIEGMSCASCVMRVEKVLKKIPGVTEVSVNLATERASIVTAGAVAPE
QLIAAIEKAGYHARAHLPEEAAPTAKTALSSWWPVALSAALSLPLVLPMLLAPLGVHWSL
PPLWQWLLATPVQFWLGWRFYHAGWGALRARSGNMDLLVALGTSAAYGLSLWQWLTLPTM
PGHAMAMPHLYFESSAVVITLVLLGKWLEGRAKRQTADAIAALNALRPENARVRRDGQDI
EVPLAQVRVGDLVVLRPGERVPVDGVVREGRSHLDEAMLTGESRPQAKQAGDPLTAGSIN
AEGVLVMETTAIGAETVLARIVRMVEQAQAAKAPIQRLVDQVSAVFVPAVLLLSLLTLLG
WGLVQGNWEQALLNAVAVMVIACPCALGLATPTAIMAGTGVAARHGILIKDAEALEIAHR
VNAVAFDKTGTLTLGHPRLLELEATPSTQPDELLALAAALQAHSEHPLGRAVREAAAQSA
QAARQAEQVRSVAGLGIEGVVEGRQLMLGSSRWMQQLQAHAPALQARAEQLQAQGRTLSW
LARRNDSGVEVLGLLAFGDQIKPGAQAAIATLHRMGVRSAMLSGDNLGAAQQVASELGIQ
EVRAEVLPADKAEAVTALRQGGAQVVAMVGDGVNDAPALAAADVGMAMSTGTDVAMQAAG
VTLMHGDPGLVADAIDISRRTYAKIRQNLFWAFIYNLVGIPLAALGLLNPMLAGAAMAFS
SVSVVSNALLLRRWRPHAPVSTKE