Protein Info for HSERO_RS07770 in Herbaspirillum seropedicae SmR1

Annotation: acetyl-CoA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02188: acetate--CoA ligase
  • amino acids 27 to 658 (632 residues), 971.1 bits, E=1.1e-296
• PF16177: ACAS_N
  • amino acids 32 to 87 (56 residues), 69 bits, 3.7e-23
• PF00501: AMP-binding
  • amino acids 95 to 480 (386 residues), 305.8 bits, E=5.9e-95
• PF13193: AMP-binding_C
  • amino acids 544 to 625 (82 residues), 96.5 bits, E=2.2e-31

Best Hits

Swiss-Prot: 81% identical to ACSA_HERAR: Acetyl-coenzyme A synthetase (acsA) from Herminiimonas arsenicoxydans

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 100% identity to hse:Hsero_1545)

MetaCyc: 79% identical to acetyl-CoA synthetase (Cupriavidus necator)
Propionate--CoA ligase. [EC: 6.2.1.17]; Acetate--CoA ligase. [EC: 6.2.1.17, 6.2.1.1]

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• 2-methylcitrate cycle I (5/5 steps found)
• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (8/10 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• ethanol degradation III (2/3 steps found)
• β-alanine biosynthesis II (4/6 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• chitin deacetylation (2/4 steps found)
• acrylate degradation II (1/3 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• cis-geranyl-CoA degradation (2/9 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.1, 6.2.1.17

Use Curated BLAST to search for 6.2.1.1 or 6.2.1.17

Sequence Analysis Tools

See D8IQ13 at UniProt or InterPro

Protein Sequence (660 amino acids)

>HSERO_RS07770 acetyl-CoA synthetase (Herbaspirillum seropedicae SmR1)
VADIETFKQENRVFAPPAELVKHAAISGMDAYHALNAEFAKDYEGTWARLAKDNLKWHKP
FTQTLDESNAPFYKWFADGQVNVSYNCLDVNLENGNADKTAVIFESDDGKVTRVSYRELH
QKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHER
VIDAGAVAVLTADYQVRGGKQLPLKSIVDEALDMGGCDTLKHVVVYKRAGAEINWVEGRD
LWLSDVVANQSDTCEPEWVDAEHPLFILYTSGSTGKPKGVQHASAGYLLWAVLTMKWTFD
IKPDDVYWCTADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIF
YTAPTAIRSLIKAADADEKVHPKQYDLSSLRILGSVGEPINPEAWMWYYKNVGHENCPIV
DTFWQTETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPW
PSMIRTIWNDPERFKKSYFPEELGGKIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRM
GTMEIESALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGDEAKQIAKELRDWVA
KEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAKGEAITQDVSTLENPAILDQLKEAQ