Protein Info for HSERO_RS07295 in Herbaspirillum seropedicae SmR1

Annotation: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 6 to 120 (115 residues), 146.1 bits, E=5.3e-47
• PF02882: THF_DHG_CYH_C
  • amino acids 123 to 280 (158 residues), 231.9 bits, E=2.3e-73

Best Hits

Swiss-Prot: 81% identical to FOLD_CUPNJ: Bifunctional protein FolD (folD) from Cupriavidus necator (strain JMP 134 / LMG 1197)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 100% identity to hse:Hsero_1451)

MetaCyc: 59% identical to methylenetetrahydrofolate dehydrogenase [multifunctional] (Bacillus methanolicus)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• folate transformations II (plants) (10/11 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
• folate transformations I (9/13 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• purine nucleobases degradation II (anaerobic) (15/24 steps found)
• L-histidine degradation III (2/6 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (3/10 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Sequence Analysis Tools

See D8IPE7 at UniProt or InterPro

Protein Sequence (281 amino acids)

>HSERO_RS07295 bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase (Herbaspirillum seropedicae SmR1)
MPAQLIDGNLLSQQLRAEVAKRAAALTAQGKQPGLAVILVGDSPASQVYVRNKVKACQDN
GLHSVLEKYEASMTEAELLARIDALNSDPKINGILVQLPLPAHIDAHKVIEAIAPEKDVD
GFHVSNAGLLMTGQPLFRPCTPYGVMKMLESIDYPVRGANAVVVGASNIVGKPQAMLLLQ
AGATVTICNSKTRDLGHHTRQADILVVATGKRNIVTADMVKPGAVVIDVGMNRDDNGKLC
GDVDFANAKDVAGWITPVPGGVGPMTITMLLVNTIEAAERC