Protein Info for HSERO_RS07140 in Herbaspirillum seropedicae SmR1

Annotation: glutamine-synthetase adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 927 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details PF03710: GlnE" amino acids 79 to 267 (189 residues), 192 bits, see alignment E=2.5e-60 amino acids 553 to 788 (236 residues), 218.2 bits, see alignment E=2.6e-68 PF08335: GlnD_UR_UTase" amino acids 294 to 438 (145 residues), 107 bits, see alignment E=1.7e-34 amino acids 810 to 905 (96 residues), 27.8 bits, see alignment E=4.6e-10

Best Hits

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 100% identity to hse:Hsero_1420)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IPB6 at UniProt or InterPro

Protein Sequence (927 amino acids)

>HSERO_RS07140 glutamine-synthetase adenylyltransferase (Herbaspirillum seropedicae SmR1)
MAAGFPSFTLCCGAFQTLMPVFSPAHHRSLVEASSSRYYTRWTQADPARPALVAAAAAAG
ALDRAFLQQRLEHDLASGLSLPAAMRRLRNLVICTLITRDLDGRGDLDEVVTTMTGFADF
AVQTHLAALMQEQTALYGLPIGEESGRPQEMIVLGMGKLGGGELNVSSDIDLIFVYPEDG
DTRAEAGQKSLSNHEFFVRLGKKLIGALAEITEDGFTFRVDMALRPNGNSGPLVASFNMV
EEYLVRQGREWERYAWTKARALTGTPEDIATLEAISRPFIFRRYLDYGSIDALRSMHGQI
RAEVKRQEALHPDRSNNVKLGRGGIREIEFTSQVFQLIRGGRDAELRDRSTRTTLRTLAA
KDLLAPEVVEQLLHAYTFLRNLEHRLQYLEDAQTHTLPVNPDDLLLVARMMGYEEPAALL
HELERQRAIVAAQFDAIFADKQSEADNDGPSVSVSDNDNLEGLADALRLVGFPEADIADG
ARRLHLTWQSPRMQSLPEASRNRLNTVINTSLPLLAALHYDQLPALGRLLDLLEAIARRA
AYLALLTEYPYALQRLVRMIGASGWAATYLTRHPLLLDELLDDRNLKAASDWAAFAENCR
RQLATAEGDTERQLDILRELHHAEQFRLLAQDLEGDLSVEKLADELSALADVLVQVTIEA
VWQTVAQRHREVPQFAVIAYGKLGGKELGYASDLDVVFLYDDEDQDAPGLYAKLAQRFIT
WMTSHTPAGTLFDIDIALRPDGASGLLVSPLSSFEKYQLNAAWIWEHQALTRARFCAGDT
AIGERFEALRERVLRQPRDPARLEEEVLSMRRRMREAHPNRSTMFDLKHDEGGMIDIEFM
VQYLVLRHASDHPQLTGDIGNIALLKLAGQLGLIDALLADEAANAYRLFRKLQHQIRLQG
SERAHIDAIRVEHERACVIRLWQQVFG