Protein Info for HSERO_RS06990 in Herbaspirillum seropedicae SmR1

Annotation: membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 517 transmembrane" amino acids 21 to 38 (18 residues), see Phobius details amino acids 51 to 69 (19 residues), see Phobius details amino acids 90 to 114 (25 residues), see Phobius details amino acids 134 to 153 (20 residues), see Phobius details amino acids 161 to 181 (21 residues), see Phobius details amino acids 187 to 208 (22 residues), see Phobius details amino acids 240 to 258 (19 residues), see Phobius details amino acids 278 to 298 (21 residues), see Phobius details amino acids 313 to 333 (21 residues), see Phobius details amino acids 354 to 375 (22 residues), see Phobius details amino acids 387 to 406 (20 residues), see Phobius details amino acids 412 to 433 (22 residues), see Phobius details amino acids 445 to 467 (23 residues), see Phobius details amino acids 483 to 507 (25 residues), see Phobius details TIGR01695: murein biosynthesis integral membrane protein MurJ" amino acids 3 to 515 (513 residues), 472.1 bits, see alignment E=1.1e-145 PF03023: MurJ" amino acids 28 to 472 (445 residues), 439.8 bits, see alignment E=5.1e-136

Best Hits

Swiss-Prot: 63% identical to MURJ_BURCJ: Lipid II flippase MurJ (murJ) from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

KEGG orthology group: K03980, virulence factor (inferred from 100% identity to hse:Hsero_1392)

MetaCyc: 53% identical to lipid II flippase MurJ (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-286

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IP88 at UniProt or InterPro

Protein Sequence (517 amino acids)

>HSERO_RS06990 membrane protein (Herbaspirillum seropedicae SmR1)
MNLHKTLAAISGMTMLSRITGLVREILFARAFGASAYTDAFNIAFRIPNLLRRLFAEGAF
SQAFVPILAEYKNQRGEQQTKHLVDHVATVLMWVMLVTCVAGILATPFVVYFIATGLQYN
PDAFNASVVMTRIMFPYIGFMAFVALAGGILNTWKEFKIPAVTSVLLNLAFIIASLFVAP
FMAQPIYAMAFAVLVGGILQVAIQVPALMKIGMLPHLYWNPLLGLRDEGVKRVLKKMGPA
IFAVSAAQISLMINTNIASRLEHGSVSWLSYADRLMEFPTALLGVALGTILLPSLSNAHA
AQDMEEYSSLLDWGLRLTFMLAMPSAVALATLSEPLTATLFHYGRFDALSVTMTGHALVA
YGVGLIGLVMVKILAPGFYAKQDIRTPVIIAVGVLIATQLMNSVFVPLFAHAGLALSIGL
GACLNALCLFIGLRRRQIYVPRPGWGLFLVRLTGALLVLAGASLWIAGHFDWIALRAHPL
MRIGALALVMLVCALAYFGPLFAMGFRLREFKRIARR