Protein Info for HSERO_RS06135 in Herbaspirillum seropedicae SmR1

Annotation: malonic semialdehyde reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 2 to 188 (187 residues), 180.4 bits, E=7.1e-57
• PF01370: Epimerase
  • amino acids 3 to 176 (174 residues), 26.3 bits, E=1.2e-09
• PF08659: KR
  • amino acids 3 to 164 (162 residues), 46 bits, E=1.4e-15
• PF13561: adh_short_C2
  • amino acids 7 to 221 (215 residues), 117.8 bits, E=1.5e-37

Best Hits

Swiss-Prot: 63% identical to YDFG_SALTY: NADP-dependent 3-hydroxy acid dehydrogenase YdfG (ydfG) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: None (inferred from 100% identity to hse:Hsero_1222)

MetaCyc: 61% identical to 3-hydroxy acid dehydrogenase YdfG (Escherichia coli K-12 substr. MG1655)
RXN-16000 [EC: 1.1.1.381]; RXN-8974 [EC: 1.1.1.381, 1.1.1.298]; Serine 3-dehydrogenase. [EC: 1.1.1.381, 1.1.1.298, 1.1.1.276]

Predicted SEED Role

"3-hydroxypropionate dehydrogenase (EC 1.1.1.298)" (EC 1.1.1.298)

MetaCyc Pathways

• superpathway of L-threonine metabolism (13/18 steps found)
• L-threonine degradation III (to methylglyoxal) (2/3 steps found)
• β-alanine degradation III (1/2 steps found)
• uracil degradation III (3/5 steps found)
• 3-hydroxypropanoate cycle (5/13 steps found)
• glyoxylate assimilation (5/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (8/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (6/18 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.276 or 1.1.1.298 or 1.1.1.381

Sequence Analysis Tools

See D8INR8 at UniProt or InterPro

Protein Sequence (250 amino acids)

>HSERO_RS06135 malonic semialdehyde reductase (Herbaspirillum seropedicae SmR1)
MVVLITGATAGFGAAMARKFAGNGHKVIAAGRRKERLDDLVKELGADKVLPLVLDVTSKS
SIETGLASLTGAWKEVDVLINNAGLALGLGPAHEVALSDWETMIDTNVKGLVTITHALLP
DMVKRGRGTIINLGSVAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAPGL
CGGTEFSNVRFKGDDGAAAKVYEGTTPLTADDIAEAAFWVATMPAHVNINYMELMPTCQG
FSPFNIKRKQ