Protein Info for HSERO_RS04765 in Herbaspirillum seropedicae SmR1

Annotation: alkanesulfonate monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 385 TIGR04021: dimethyl sulfone monooxygenase SfnG" amino acids 17 to 366 (350 residues), 643.4 bits, see alignment E=5.5e-198 PF00296: Bac_luciferase" amino acids 18 to 337 (320 residues), 244.2 bits, see alignment E=1.1e-76

Best Hits

Swiss-Prot: 79% identical to SFNG_PSEPF: FMNH(2)-dependent dimethylsulfone monooxygenase (sfnG) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K04091, alkanesulfonate monooxygenase [EC: 1.14.14.5] (inferred from 100% identity to hse:Hsero_0951)

MetaCyc: 79% identical to dimethylsulfone monooxygenase (Pseudomonas fluorescens Pf0-1)
RXN-14709 [EC: 1.14.14.35]

Predicted SEED Role

"Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases; sulfonate monooxygenase"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.14.14.5

Use Curated BLAST to search for 1.14.14.35 or 1.14.14.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8J0R3 at UniProt or InterPro

Protein Sequence (385 amino acids)

>HSERO_RS04765 alkanesulfonate monooxygenase (Herbaspirillum seropedicae SmR1)
MSQAPSTTAAATRTDAVKFAYWVPNVSGGLVVSTIPQRTDWSLEYNQRLAVTAEKAGFEY
ALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILPGPWHPAVVAKQIATIDHIS
NGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRALKGIWTQDDFTFKGDFYRFN
NYTLSPKPVQKPHPEIFQGGSSRAARDNAASVSDWYFTNGNTLEGLKAQIDDIQAKAAQN
GHKVRIGVNAFVIARDTEEEAQAVLQDIIDHAHVEAVHAFGDAVKQAGKASPEGEGNWAK
STFEDLVQYNDGFKTNLIGTPQQIAERIVALKAIGVDLVLTGFLHFIEEVEYFGQKVLPL
VRELEARQAATAASPAAAPVLEAVA