Protein Info for HSERO_RS03650 in Herbaspirillum seropedicae SmR1

Updated annotation (from data): Mannose isomerase (EC 5.3.1.7)
Rationale: Specifically important for utilizing D-Mannose. Automated validation from mutant phenotype: the predicted function (5.3.1.7) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: N-acyl-D-glucosamine 2-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07221: GlcNAc_2-epim
  • amino acids 33 to 384 (352 residues), 282.2 bits, E=3.3e-88

Best Hits

KEGG orthology group: None (inferred from 100% identity to hse:Hsero_0730)

Predicted SEED Role

"D-mannose isomerase (EC 5.3.1.7)" in subsystem Mannose Metabolism (EC 5.3.1.7)

KEGG Metabolic Maps

• Fructose and mannose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.3.1.7

Sequence Analysis Tools

See D8IZC9 at UniProt or InterPro

Protein Sequence (396 amino acids)

>HSERO_RS03650 Mannose isomerase (EC 5.3.1.7) (Herbaspirillum seropedicae SmR1)
MPQPDFESRRFLHAHVADTLAFYDRHAIDPLGGFFHYLKDNGRVYNAGHRHLVSATRLVF
TQTMAYTHSGHTRYLRQARRAWDELARFRHASGPLQGLYAWTLQDERIEDATVMAYGQAF
VLLACAHAHRIGLCGEDAVADAFERMETAFFEPAHQAYADEITPAGELIGYRGQNANMHM
CEACLSAYEVTGQRRYLERALALIERFVFDLAQHTQGLVWEHYHQDWTPDLDYNKGKRSN
IFKPWGFQTGHQTEWAKLLLIAHGHAPNAAFLPRAIELHQAAWTYGWDSIHGGLIYGFGL
EREPYDRDKYFWVQAESFASAWRLWRATGEPAFRDQYRMLWAWSWQHMVDHEHGAWFRIL
AADGSKLEDTKSPAGKVDYHTMGACWDVLAVGGLGV