Protein Info for HSERO_RS03615 in Herbaspirillum seropedicae SmR1

Annotation: (dimethylallyl)adenosine tRNA methylthiotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 4 to 440 (437 residues), 478.6 bits, see alignment E=1.7e-147 TIGR01574: tRNA-i(6)A37 thiotransferase enzyme MiaB" amino acids 4 to 443 (440 residues), 594.1 bits, see alignment E=1.9e-182 PF00919: UPF0004" amino acids 4 to 104 (101 residues), 104.1 bits, see alignment E=4.9e-34 PF04055: Radical_SAM" amino acids 151 to 325 (175 residues), 93.2 bits, see alignment E=3.1e-30 PF01938: TRAM" amino acids 379 to 443 (65 residues), 54 bits, see alignment E=1.9e-18

Best Hits

Swiss-Prot: 80% identical to MIAB_JANMA: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (miaB) from Janthinobacterium sp. (strain Marseille)

KEGG orthology group: K06168, bifunctional enzyme involved in thiolation and methylation of tRNA (inferred from 100% identity to hse:Hsero_0723)

MetaCyc: 62% identical to isopentenyl-adenosine A37 tRNA methylthiolase (Escherichia coli K-12 substr. MG1655)
RXN0-5063 [EC: 2.8.4.3]

Predicted SEED Role

"tRNA-i(6)A37 methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase or tRNA processing

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IZC2 at UniProt or InterPro

Protein Sequence (446 amino acids)

>HSERO_RS03615 (dimethylallyl)adenosine tRNA methylthiotransferase (Herbaspirillum seropedicae SmR1)
MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF
SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY
SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED
VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH
PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI
TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA
RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR
LIGQIIEVSITEAFPFSLRGEIVTQH