Protein Info for HSERO_RS03540 in Herbaspirillum seropedicae SmR1

Annotation: dimethyladenosine transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 260 TIGR00755: ribosomal RNA small subunit methyltransferase A" amino acids 5 to 255 (251 residues), 270.1 bits, see alignment E=8.9e-85 PF00398: RrnaAD" amino acids 6 to 256 (251 residues), 218.1 bits, see alignment E=6.1e-69

Best Hits

Swiss-Prot: 81% identical to RSMA_JANMA: Ribosomal RNA small subunit methyltransferase A (rsmA) from Janthinobacterium sp. (strain Marseille)

KEGG orthology group: K02528, 16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase [EC: 2.1.1.182] (inferred from 100% identity to hse:Hsero_0708)

Predicted SEED Role

"SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase (EC 2.1.1.182)" (EC 2.1.1.182)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.182

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See D8IZA7 at UniProt or InterPro

Protein Sequence (260 amino acids)

>HSERO_RS03540 dimethyladenosine transferase (Herbaspirillum seropedicae SmR1)
MKHIPRKRFGQNFLTDEMVLHDIITSIAPAADDAMVEIGPGLAAMTALLLEGLRHLHVVE
LDRDLVERLKKRFDAARLTVHSADALKFDFSTIPVPEGRKLRVVGNLPYNISSPLLFHLA
EIAPQVQDQHFMLQKEVVERMVAEPGSKAYGRLSVMLQWRYHMELLFVVPPTAFDPPPKV
DSAIVRMIPLAQPLACQQALLEQVVTKAFSQRRKVIRNCVAGLFSEDELRQVGIDPQARP
EAVPMEQFVALANLLAARQH